Document Detail


Metabolic profiling detects biomarkers of protein degradation in COPD patients.
MedLine Citation:
PMID:  22183483     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
There is a paucity of biomarkers for chronic obstructive pulmonary disease (COPD). Metabolomics were applied to a defined COPD patient cohort from the ECLIPSE study (Evaluation of COPD Longitudinally to Identify Predictive Surrogate End-points). Results were correlated with accepted biomarkers for the disease. Baseline control serum (n=66) and Global Initiative for Chronic Obstructive Lung Disease (GOLD) stage II (n=70), III (n=64) and IV (n=44) COPD patients were analysed by proton nuclear magnetic resonance ((1)H NMR). Liquid chromatography with tandem mass spectrometry (LC-MS/MS) was used to confirm amino acid changes detected by (1)H NMR. Data were correlated with body composition, emphysema and systemic inflammation. (1)H NMR identified decreased lipoproteins, N,N-dimethylglycine, and increased glutamine, phenylalanine, 3-methylhistidine and ketone bodies in COPD patients with decreased branched-chain amino acids (BCAAs) observed in GOLD stage IV patients. BCAAs, their degradation products, 3-methylhistidine, ketone bodies, and triglycerides were correlated negatively with cachexia and positively with systemic inflammation. Emphysema patients also displayed decreased serum creatine, glycine and N,N-dimethylglycine. LC-MS/MS confirmed (1)H NMR findings relating to BCAAs, glutamine and 3-methylhistidine in GOLD stage IV patients. NMR-based metabolomics characterised COPD patients based on systemic effects and lung function parameters. Increased protein turnover occurred in all COPD patients with increased protein degradation in individuals with emphysema and cachexia.
Authors:
Baljit K Ubhi; John H Riley; Paul A Shaw; David A Lomas; Ruth Tal-Singer; William MacNee; Julian L Griffin; Susan C Connor
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-12-19
Journal Detail:
Title:  The European respiratory journal     Volume:  40     ISSN:  1399-3003     ISO Abbreviation:  Eur. Respir. J.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-02     Completed Date:  2013-01-10     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  8803460     Medline TA:  Eur Respir J     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  345-55     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Amino Acids / chemistry
Biological Markers / metabolism*
Body Mass Index
Chromatography, Liquid / methods
Cohort Studies
Female
Humans
Longitudinal Studies
Magnetic Resonance Spectroscopy / methods
Male
Mass Spectrometry / methods
Metabolomics / methods
Middle Aged
Mitochondria / metabolism
Nutritional Status
Proteins / metabolism*
Proteolysis
Pulmonary Disease, Chronic Obstructive / diagnosis,  metabolism*
Smoking / adverse effects
Grant Support
ID/Acronym/Agency:
G0901697//Medical Research Council; G0901786//Medical Research Council
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Biological Markers; 0/Proteins

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