Document Detail

Metabolic profiling of bile in cholangiocarcinoma using in vitro magnetic resonance spectroscopy.
MedLine Citation:
PMID:  20662790     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: Cholangiocarcinoma (CCA) has a poor prognosis and its aetiology is inadequately understood. Magnetic resonance spectroscopy (MRS) of bile may provide insights into the pathogenesis of CCA and help identify novel diagnostic biomarkers. The aim of this study was to compare the chemical composition of bile from patients with CCA with that of bile from patients with benign biliary disease.
METHODS: Magnetic resonance spectra were acquired from the bile of five CCA patients and compared with MRS of control bile from patients with benign biliary disease (seven with gallstones, eight with sphincter of Oddi dysfunction [SOD], five with primary sclerosing cholangitis [PSC]). Metabolic profiles were compared using both univariate and multivariate pattern-recognition analysis.
RESULTS: Univariate analysis showed that levels of glycine-conjugated bile acids were significantly increased in patients with CCA, compared with the benign disease groups (P= 0.002). 7 beta primary bile acids were significantly increased (P= 0.030) and biliary phosphatidylcholine (PtC) levels were reduced (P= 0.010) in bile from patients with CCA compared with bile from gallstone patients. These compounds were also of primary importance in the multivariate analysis: the cohorts were differentiated by partial least squares discriminant analysis (PLS-DA).
CONCLUSIONS: These preliminary data suggest that altered bile acid and PtC metabolism play an important role in CCA aetiopathogenesis and that specific metabolites may have potential as future biomarkers.
Amar W Sharif; Horace R T Williams; Temi Lampejo; Shahid A Khan; Devinder S Bansi; David Westaby; Andrew V Thillainayagam; Howard C Thomas; I Jane Cox; Simon D Taylor-Robinson
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  HPB : the official journal of the International Hepato Pancreato Biliary Association     Volume:  12     ISSN:  1477-2574     ISO Abbreviation:  HPB (Oxford)     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-28     Completed Date:  2011-03-25     Revised Date:  2011-08-03    
Medline Journal Info:
Nlm Unique ID:  100900921     Medline TA:  HPB (Oxford)     Country:  England    
Other Details:
Languages:  eng     Pagination:  396-402     Citation Subset:  IM    
Hepatology and Gastroenterology Section, Department of Medicine, Imperial College London, London, UK.
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MeSH Terms
Aged, 80 and over
Bile / metabolism*
Bile Acids and Salts / metabolism
Bile Duct Neoplasms / metabolism*,  pathology
Bile Ducts, Intrahepatic / metabolism*,  pathology
Case-Control Studies
Cholangiocarcinoma / metabolism*,  pathology
Glycine / analogs & derivatives,  metabolism
Magnetic Resonance Spectroscopy*
Metabolomics / methods*
Middle Aged
Multivariate Analysis
Pattern Recognition, Automated
Phosphatidylcholines / metabolism
Principal Component Analysis
Tumor Markers, Biological / metabolism*
Reg. No./Substance:
0/Bile Acids and Salts; 0/Phosphatidylcholines; 0/Tumor Markers, Biological; 56-40-6/Glycine

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