Document Detail


Metabolic profiling of a Rhizopus oryzae fumaric acid production mutant generated by femtosecond laser irradiation.
MedLine Citation:
PMID:  22516248     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Femtosecond laser irradiation was employed to induce mutations in Rhizopus oryzae, leading to increases in fumaric acid production. Compared to the parental strain, mutant strain FM19 exhibited an increase in titer and yield of 56.3% and 36.6%, respectively, corresponding to a titer of 49.4g/L and a yield of 0.56g fumaric acid per g glucose. Metabolic profiling by gas chromatography-mass spectrometry revealed that higher levels of carbon (Embden-Meyerhof-Parnas and tricarboxylic acid cycle) and amino acid metabolism were operating in the high-yielding strain; particularly, 4-aminobutyric acid and 5-aminolevulinic acid were increased 10.33- and 7.22-fold, respectively, compared with parental strain during stationary phase. These findings provided new insights into metabolic characterization of high-yielding fumaric acid R. oryzae.
Authors:
Shouzhi Yu; Di Huang; Jianping Wen; Shuang Li; Yunli Chen; Xiaoqiang Jia
Related Documents :
17051918 - Effect of some anions on the mobility of amino acids in soils by thin layer chromatogra...
6509078 - Molecular species of monoglycosylceramides in embryonic and adult japanese quail intest...
3957918 - Characterization of a diphosphonopentaosylceramide containing 3-o-methylgalactose from ...
7260738 - Streptomyces setonii: catabolism of vanillic acid via guaiacol and catechol.
582968 - Chemical characterization of decomposition products of pyrazolidinedione-3,5 derivative...
6691998 - Age-dependent responses of the serum non-esterified fatty acids to adrenalectomy and ov...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-2
Journal Detail:
Title:  Bioresource technology     Volume:  -     ISSN:  1873-2976     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9889523     Medline TA:  Bioresour Technol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Biological Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Direct lactic acid fermentation of Jerusalem artichoke tuber extract using Lactobacillus paracasei w...
Next Document:  Fungal pretreatment of cornstalk with Phanerochaete chrysosporium for enhancing enzymatic saccharifi...