Document Detail

Metabolic flux measurements across portal drained viscera, liver, kidney and hindquarter in mice.
MedLine Citation:
PMID:  11201286     Owner:  NLM     Status:  MEDLINE    
A method was developed to measure metabolic fluxes across either portally-drained viscera (PDV) and liver or kidney and hindquarter (HQ) in anesthetized mice. The method includes a primed-constant infusion of ketamine-medetomidine anaesthesia to stabilize the mice for the surgical procedures. For measurement of metabolic fluxes across PDV and liver, blood sampling catheters were inserted in the carotid artery, portal vein and hepatic vein and infusion catheters in the jugular vein and mesenteric vein. For measurement of metabolic flux across kidney and HQ, blood sampling catheters were inserted in the carotid artery, renal vein and caval vein and infusion catheters in the jugular vein and abdominal aorta. 14C-PAH was infused to enable plasma flow measurement using an indicator dilution method. In addition, we developed a blood sampling procedure without waste of blood. We measured plasma flow and metabolic fluxes across PDV, liver, kidney and HQ. Mean plasma flow in post-absorptive mice was: PDV: 0.9+/-0.2, liver: 1.2+/-0.3, kidney: 1.0+/-0.1, HQ: 1.1+/-0.3 ml/10 g body weight (b.w.)/min. Significant glutamine release by the HQ and uptake of glutamine by the kidney and PDV was observed. In PDV, citrulline is produced from glutamine and is in turn used by the kidney for the production of arginine. In conclusion, the described model enables measurement of metabolic fluxes across PDV, liver, kidney and HQ in mice. The availability of such a small animal model allows the potential for measuring metabolic parameters in transgenic and knockout mice, and therefore may lead to an important refinement in metabolic research.
M M Hallemeesch; G A Ten Have; N E Deutz
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Laboratory animals     Volume:  35     ISSN:  0023-6772     ISO Abbreviation:  Lab. Anim.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-01-26     Completed Date:  2001-03-15     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0112725     Medline TA:  Lab Anim     Country:  England    
Other Details:
Languages:  eng     Pagination:  101-10     Citation Subset:  IM    
Department of Surgery, Maastricht University, The Netherlands.
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MeSH Terms
Amino Acids / metabolism
Arginine / metabolism
Blood Flow Velocity
Catheterization / methods
Citrulline / metabolism
Glutamine / metabolism
Hindlimb / metabolism*
Kidney / metabolism*
Liver / metabolism*
Models, Animal
Portal System / metabolism*
Regional Blood Flow
Specific Pathogen-Free Organisms
Viscera / metabolism*
Reg. No./Substance:
0/Amino Acids; 372-75-8/Citrulline; 56-85-9/Glutamine; 74-79-3/Arginine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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