| Metabolic flux and cell cycle analysis indicating new mechanism underlying process oscillation in continuous ethanol fermentation with Saccharomyces cerevisiae under VHG conditions. | |
| | |
MedLine Citation:
|
PMID: 19463939 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Process oscillation characterized by long oscillation period and large oscillation amplitude was observed in continuous ethanol fermentation with Saccharomyces cerevisiae under very high gravity conditions. Metabolic flux analysis was applied to the fermentation system, and the results indicated that carbon flux distributions at the metabolic notes oscillated, correspondingly, and the root reason for the process oscillation was the intracellular metabolism of yeast cells. Cell cycle analysis with the flow cytometry showed that no cell-cycle-dependent synchronization of the daughter and mother cells occurred within the duration of the oscillation, and thus different mechanism existed compared with the oscillation observed in the continuous culture of Saccharomyces cerevisiae and triggered by the synchronization of the daughter and mother cells under specific conditions. Furthermore, the overall metabolic activity of the yeast cells was examined, which was found not exactly out of phase but lag behind ethanol concentration that accumulated within the fermentation system and its inhibition on the yeast cells as well, which supported the mechanistic speculation for the process oscillation: the lag response of yeast cells to ethanol inhibition. |
| | |
Authors:
|
Y Shen; X Q Zhao; X M Ge; F W Bai |
Related Documents
:
|
1487489 - Dolichol delays g1-arrest for one cell cycle in human fibroblasts subjected to depletio... 434029 - Proliferation kinetics of human endometrium during the normal menstrual cycle. 20870879 - Reorganization of the growth pattern of schizosaccharomyces pombe in invasive filament ... 7547499 - The nonstructural proteins of the autonomous parvovirus minute virus of mice interfere ... 18774719 - Circular reasoning: micrornas and cell-cycle control. 19589379 - Expression of the neural specific protein, gap-43, dramatically lengthens the cell cycl... 22298769 - Role of epithelial-stem cell interactions during dental cell differentiation. 6729049 - The cell cycle dependence of thermotolerance. ii. cho cells heated at 45.0 degrees c. 21468979 - Raman micro-spectroscopy as a non-invasive cell viability test. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-05-19 |
Journal Detail:
|
Title: Biotechnology advances Volume: 27 ISSN: 1873-1899 ISO Abbreviation: Biotechnol. Adv. Publication Date: 2009 Nov-Dec |
Date Detail:
|
Created Date: 2009-10-12 Completed Date: 2010-01-11 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8403708 Medline TA: Biotechnol Adv Country: England |
Other Details:
|
Languages: eng Pagination: 1118-23 Citation Subset: IM |
Affiliation:
|
Department of Bioscience and Bioengineering, Dalian University of Technology, Dalian 116023, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Cycle* Ethanol / metabolism* Fermentation Saccharomyces cerevisiae / cytology, metabolism* |
| Chemical | |
Reg. No./Substance:
|
64-17-5/Ethanol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: 25 years of recombinant proteins from reactor-grown cells - where do we go from here?
Next Document: Genome shuffling: Progress and applications for phenotype improvement.