Document Detail

Metabolic flux analysis in complex isotopolog space. Recycling of glucose in tobacco plants.
MedLine Citation:
PMID:  15680989     Owner:  NLM     Status:  MEDLINE    
Tobacco plants grown in vitro were supplied with a mixture of [U-13C6]glucose and unlabelled sucrose via the root system. After 20 days, leaves were harvested and extracted with water. Glucose was isolated from the extract and was analysed by 13C NMR spectroscopy. All 13C signals appeared as complex multiplets due to 13C-13C coupling. The abundance of 21 isotopologous glucose species was determined from the 13C NMR signal integrals by numerical deconvolution using a genetic algorithm. The relative fractions of specific isotopologs in the overall excess of 13C-labelled specimens establish flux contributions via glycolysis/glucogenesis, pentose phosphate pathway, citric acid cycle and Calvin cycle including 13CO2 refixation. The fluxes were modelled and reconstructed in silico by a novel rule-based approach yielding the contributions of circular pathways and the degree of multiple cycling events. The data indicate that the vast majority of the proffered [U-13C6]glucose molecules had been modified by catabolism and subsequent glucogenesis from catabolic fragments, predominantly via passage through the citric acid cycle and the pentose phosphate pathway.
Christian Ettenhuber; Tanja Radykewicz; Waltraud Kofer; Hans-Ulrich Koop; Adelbert Bacher; Wolfgang Eisenreich
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Phytochemistry     Volume:  66     ISSN:  0031-9422     ISO Abbreviation:  Phytochemistry     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-01-31     Completed Date:  2005-04-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0151434     Medline TA:  Phytochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  323-35     Citation Subset:  IM    
Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstr. 4, D-85747 Garching, Germany.
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MeSH Terms
Carbon / metabolism
Carbon Isotopes
Glucose / chemistry,  metabolism*
Magnetic Resonance Spectroscopy
Plant Leaves / chemistry,  metabolism
Tobacco / chemistry,  metabolism*
Zea mays / chemistry,  metabolism
Reg. No./Substance:
0/Carbon Isotopes; 50-99-7/Glucose; 7440-44-0/Carbon

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