Document Detail


Metabolic flux analysis in complex isotopolog space. Recycling of glucose in tobacco plants.
MedLine Citation:
PMID:  15680989     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tobacco plants grown in vitro were supplied with a mixture of [U-13C6]glucose and unlabelled sucrose via the root system. After 20 days, leaves were harvested and extracted with water. Glucose was isolated from the extract and was analysed by 13C NMR spectroscopy. All 13C signals appeared as complex multiplets due to 13C-13C coupling. The abundance of 21 isotopologous glucose species was determined from the 13C NMR signal integrals by numerical deconvolution using a genetic algorithm. The relative fractions of specific isotopologs in the overall excess of 13C-labelled specimens establish flux contributions via glycolysis/glucogenesis, pentose phosphate pathway, citric acid cycle and Calvin cycle including 13CO2 refixation. The fluxes were modelled and reconstructed in silico by a novel rule-based approach yielding the contributions of circular pathways and the degree of multiple cycling events. The data indicate that the vast majority of the proffered [U-13C6]glucose molecules had been modified by catabolism and subsequent glucogenesis from catabolic fragments, predominantly via passage through the citric acid cycle and the pentose phosphate pathway.
Authors:
Christian Ettenhuber; Tanja Radykewicz; Waltraud Kofer; Hans-Ulrich Koop; Adelbert Bacher; Wolfgang Eisenreich
Related Documents :
4074339 - Glycolytic origin of alanine formed in rat diaphragm muscle in vitro.
238389 - Extemporaneous preparation of methyldopa in two syrup vehicles.
6884839 - Determination of long-chain nonesterified fatty acids in sera of young women in differe...
15641759 - Assessment of enamel erosion and protective effect of salivary pellicle by surface roug...
21592789 - Indole 5-carboxamide thumb pocket i inhibitors of hcv ns5b polymerase with nanomolar po...
18464869 - Development of molecular markers linked to a gene controlling fruit acidity in citrus.
9208249 - Ascorbic acid enhances hydroxyl radical formation in iron-fortified infant cereals and ...
16343829 - Citric acid prolongs the gastro-retention of a floating dosage form and increases bioav...
20721309 - Synthesis of 1-(substituted phenylcarbonyl/sulfonylamino)-1,2,3,6-tetrahydropyridine-5-...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Phytochemistry     Volume:  66     ISSN:  0031-9422     ISO Abbreviation:  Phytochemistry     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-01-31     Completed Date:  2005-04-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0151434     Medline TA:  Phytochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  323-35     Citation Subset:  IM    
Affiliation:
Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstr. 4, D-85747 Garching, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Algorithms
Carbon / metabolism
Carbon Isotopes
Glucose / chemistry,  metabolism*
Isomerism
Magnetic Resonance Spectroscopy
Plant Leaves / chemistry,  metabolism
Tobacco / chemistry,  metabolism*
Zea mays / chemistry,  metabolism
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 50-99-7/Glucose; 7440-44-0/Carbon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Visible light-induced oxidation of unsaturated components of cutins: a significant process during th...
Next Document:  Seasonal trends in usnic acid concentrations of Arctic, alpine and Patagonian populations of the lic...