Document Detail


Metabolic fate of oleic acid, palmitic acid and stearic acid in cultured hamster hepatocytes.
MedLine Citation:
PMID:  8670161     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Unlike other saturated fatty acids, dietary stearic acid does not appear to raise plasma cholesterol. The reason for this remains to be established, although it appears that it must be related to inherent differences in the metabolism of the fatty acid. In the present study, we have looked at the metabolism of palmitic acid and stearic acid, in comparison with oleic acid, by cultured hamster hepatocytes. Stearic acid was taken up more slowly and was poorly incorporated into both cellular and secreted triacylglycerol. Despite this, stearic acid stimulated the synthesis and secretion of triacylglycerol to the same extent as the other fatty acids. Incorporation into cellular phospholipid was lower for oleic acid than for palmitic acid and stearic acid. Desaturation of stearic acid, to monounsaturated fatty acid, was found to be greater than that of palmitic acid. Oleic acid produced from stearic acid was incorporated into both triacylglycerol and phospholipid, representing 13% and 6% respectively of the total after a 4 h incubation. Significant proportions of all of the fatty acids were oxidized, primarily to form ketone bodies, but by 8 h more oleic acid had been oxidized compared with palmitic acid and stearic acid.
Authors:
J S Bruce; A M Salter
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  316 ( Pt 3)     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1996 Jun 
Date Detail:
Created Date:  1996-08-07     Completed Date:  1996-08-07     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  847-52     Citation Subset:  IM    
Affiliation:
Department of Applied Biochemistry and Food Science, University of Nottingham, Loughborough, Leicestershire, U.K.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Carbon Radioisotopes
Cells, Cultured
Cricetinae
Fatty Acids, Monounsaturated / metabolism
Fatty Acids, Nonesterified / isolation & purification,  metabolism
Ketone Bodies / metabolism
Kinetics
Liver / metabolism*
Male
Mesocricetus
Oleic Acid
Oleic Acids / metabolism*
Oxidation-Reduction
Palmitic Acid
Palmitic Acids / metabolism*
Radioisotope Dilution Technique
Stearic Acids / metabolism*
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Fatty Acids, Monounsaturated; 0/Fatty Acids, Nonesterified; 0/Ketone Bodies; 0/Oleic Acids; 0/Palmitic Acids; 0/Stearic Acids; 112-80-1/Oleic Acid; 57-10-3/Palmitic Acid; 57-11-4/stearic acid
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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