Document Detail


Metabolic fate of intravenously administered N-acetylneuraminic acid-6-14C in newborn piglets.
MedLine Citation:
PMID:  17215187     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Sialic acid (N-acetylneuraminic acid), a component of gangliosides and sialylglycoproteins, may be a conditional nutrient in early life because endogenous synthesis is limited. The aim of this study was to investigate the metabolic fate of intravenously administrated N-acetylneuraminic acid-6-14C (sialic acid) in piglets. METHOD: Three-day-old male domestic piglets (Sus scrofa) were injected via the jugular vein with 5 microCi (11-12 x 10(6) cpm) of N-acetylneuraminic acid-6-14C (specific activity of 55 mCi/mmol). Blood samples were collected at regular intervals over the next 120 min. The organs were then removed and the urine collected for determination of residual radioactivity. RESULTS: Within 2 min of injection, 80% of the activity was removed from the blood and by 120 min the remaining activity approached 8%. At 120 min, the brain contained significantly more radioactivity (cpm/g tissue) than the liver, pancreas, heart and spleen, but less than the kidneys. Within the brain, the percentage of total injected activity was highest in the cerebrum (0.175 +/-0.008) followed by the cerebellum (0.0295 +/-0.006, p=0.00006) and the thalamus (0.029 +/- 0.006, p =0.00003). CONCLUSIONS: An exogenous source of sialic acid is capable of crossing the blood-brain barrier and being taken up into various tissues. The findings suggest that dietary sources of sialic acid may contribute to early brain development in newborn mammals.
Authors:
Bing Wang; Jeff A Downing; Peter Petocz; Jennie Brand-Miller; Wayne L Bryden
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Asia Pacific journal of clinical nutrition     Volume:  16     ISSN:  0964-7058     ISO Abbreviation:  Asia Pac J Clin Nutr     Publication Date:  2007  
Date Detail:
Created Date:  2007-01-11     Completed Date:  2007-04-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9440304     Medline TA:  Asia Pac J Clin Nutr     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  110-5     Citation Subset:  IM    
Affiliation:
Human Nutrition Unit, School of Molecular and Microbial Biosciences, G08 University of Sydney, NSW 2006, Australia. B.Wang@mmb.usyd.edu.au
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn / metabolism
Brain / metabolism*
Carbon Radioisotopes / diagnostic use
Infusions, Intravenous
Kidney / metabolism
Liver / metabolism
Male
N-Acetylneuraminic Acid / pharmacokinetics*
Organ Specificity
Spleen / metabolism
Sus scrofa / blood,  metabolism*,  urine
Tissue Distribution
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 131-48-6/N-Acetylneuraminic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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