Document Detail


Metabolic engineering of omega3-very long chain polyunsaturated fatty acid production by an exclusively acyl-CoA-dependent pathway.
MedLine Citation:
PMID:  18556660     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
omega3-Very long chain polyunsaturated fatty acids (VLCPUFA) are essential for human development and brain function and, thus, are indispensable components of the human diet. The current main source of VLCPUFAs is represented by ocean fish stocks, which are in severe decline, and the development of alternative, sustainable sources of VLCPUFAs is urgently required. Our research aims at exploiting the powerful infrastructure available for the large scale culture of oilseed crops, such as rapeseed, to produce VLCPUFAs such as eicosapentaenoic acid in transgenic plants. VLCPUFA biosynthesis requires repeated desaturation and repeated elongation of long chain fatty acid substrates. In previous experiments the production of eicosapentaenoic acid in transgenic plants was found to be limited by an unexpected bottleneck represented by the acyl exchange between the site of desaturation, endoplasmic reticulum-associated phospholipids, and the site of elongation, the cytosolic acyl-CoA pool. Here we report on the establishment of a coordinated, exclusively acyl-CoA-dependent pathway, which avoids the rate-limiting transesterification steps between the acyl lipids and the acyl-CoA pool during VLCPUFA biosynthesis. The pathway is defined by previously uncharacterized enzymes, encoded by cDNAs isolated from the microalga Mantoniella squamata. The conceptual enzymatic pathway was established and characterized first in yeast to provide proof-of-concept data for its feasibility and subsequently in seeds of Arabidopsis thaliana. The comparison of the acyl-CoA-dependent pathway with the known lipid-linked pathway for VLCPUFA biosynthesis showed that the acyl-CoA-dependent pathway circumvents the bottleneck of switching the Delta6-desaturated fatty acids between lipids and acyl-CoA in Arabidopsis seeds.
Authors:
Mareike Hoffmann; Martin Wagner; Amine Abbadi; Martin Fulda; Ivo Feussner
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-13
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  283     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-08-11     Completed Date:  2008-10-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  22352-62     Citation Subset:  IM    
Affiliation:
Albrecht-von-Haller-Institut für Pflanzenwissenschaften, Abteilung Biochemie der Pflanze, Justus-von-Liebig-Weg 11, D-37077 Göttingen, Germany.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AM949596;  AM949597
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MeSH Terms
Descriptor/Qualifier:
Acyl Coenzyme A / metabolism*
Animals
Cloning, Molecular
DNA Primers
DNA, Complementary / genetics
Euglena / enzymology
Fatty Acids, Unsaturated / biosynthesis*
Gene Library
Humans
Mammals
Molecular Sequence Data
Nutritional Status
Restriction Mapping
Chemical
Reg. No./Substance:
0/Acyl Coenzyme A; 0/DNA Primers; 0/DNA, Complementary; 0/Fatty Acids, Unsaturated

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