Document Detail

Metabolic engineering of glutamate production.
MedLine Citation:
PMID:  12523388     Owner:  NLM     Status:  MEDLINE    
Since the discovery of Corynebacterium glutamicum as an efficient glutamate-overproducing microorganism in 1957, the production of L-amino acids by the fermentative method has become one of the most important research-target of industrial microbiology. Several research groups have developed metabolic engineering principles for L-amino acid-producing C. glutamicum strains over the last four decades. The mechanism of L-glutamate-overproduction by the microorganism is very unique and interesting. L-Glutamate overproduction by this bacterium, a biotin auxotroph, is induced by a biotin limitation and suppressed by an excess of biotin. Addition of a surfactant or penicillin is known to induce L-glutamate overproduction under excess biotin. After the development of the general molecular biology tools such as cloning vectors and DNA transfer technique, genes encoding biosynthetic enzymes were isolated. With those genes and tools, recombinant DNA technology can be applied in analysis of biosynthetic pathways and strain construction of C. glutamicum. In this review, key points of the L-glutamate biosynthetic pathways are summarized and the recent studies about triggering mechanism of L-glutamate overproduction by C. glutamicum are introduced. Then the metabolic flux analysis of L-glutamate overproduction is explored.
Eiichiro Kimura
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Advances in biochemical engineering/biotechnology     Volume:  79     ISSN:  0724-6145     ISO Abbreviation:  Adv. Biochem. Eng. Biotechnol.     Publication Date:  2003  
Date Detail:
Created Date:  2003-01-13     Completed Date:  2003-02-14     Revised Date:  2005-11-16    
Medline Journal Info:
Nlm Unique ID:  8307733     Medline TA:  Adv Biochem Eng Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  37-57     Citation Subset:  IM    
Fermentation & Biotechnology Laboratories, Ajinomoto Co., Inc., 1-1 suzuki-cho, Kawasaki-ku, Kawasaki-shi, 210-8681 Japan.
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MeSH Terms
Cloning, Molecular
Corynebacterium / genetics*,  metabolism*
Gene Expression Regulation, Bacterial
Glutamic Acid / biosynthesis*,  genetics*
Protein Engineering / methods*
Recombination, Genetic*
Reg. No./Substance:
56-86-0/Glutamic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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