| Metabolic engineering of Escherichia coli for 1-butanol biosynthesis through the inverted aerobic fatty acid β-oxidation pathway. | |
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MedLine Citation:
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PMID: 22105550 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The basic reactions of the clostridial 1-butanol biosynthesis pathway can be regarded to be the inverted reactions of the fatty acid β-oxidation pathway. A pathway for the biosynthesis of fuels and chemicals was recently engineered by combining enzymes from both aerobic and anaerobic fatty acid β-oxidation as well as enzymes from other metabolic pathways. In the current study, we demonstrate the inversion of the entire aerobic fatty acid β-oxidation cycle for 1-butanol biosynthesis. The constructed markerless and plasmidless Escherichia coli strain BOX-3 (MG1655 lacI ( Q ) attB-P( trc-ideal-4)-SD(φ10)-adhE(Glu568Lys) attB-P( trc-ideal-4)-SD(φ10)-atoB attB-P( trc-ideal-4)-SD(φ10)-fadB attB-P( trc-ideal-4)-SD(φ10)-fadE) synthesises 0.3-1 mg 1-butanol/l in the presence of the specific inducer. No 1-butanol production was detected in the absence of the inducer. |
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Authors:
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Andrey Yu Gulevich; Alexandra Yu Skorokhodova; Alexey V Sukhozhenko; Rustem S Shakulov; Vladimir G Debabov |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-22 |
Journal Detail:
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Title: Biotechnology letters Volume: - ISSN: 1573-6776 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Research Institute for Genetics and Selection of Industrial Microorganisms, 1-st Dorozhniy Pr., 1, Moscow, Russia, 117545, gulevich@genetika.ru. |
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