Document Detail

Metabolic engineering of Clostridium acetobutylicum for the industrial production of 1,3-propanediol from glycerol.
MedLine Citation:
PMID:  16095939     Owner:  NLM     Status:  MEDLINE    
Clostridium butyricum is to our knowledge the best natural 1,3-propanediol producer from glycerol and the only microorganism identified so far to use a coenzyme B12-independent glycerol dehydratase. However, to develop an economical process of 1,3-propanediol production, it would be necessary to improve the strain by a metabolic engineering approach. Unfortunately, no genetic tools are currently available for C. butyricum and all our efforts to develop them have been so far unsuccessful. To obtain a better "vitamin B12-free" biological process, we developed a metabolic engineering strategy with Clostridium acetobutylicum. The 1,3-propanediol pathway from C. butyricum was introduced on a plasmid in several mutants of C. acetobutylicum altered in product formation. The DG1(pSPD5) recombinant strain was the most efficient strain and was further characterized from a physiological and biotechnological point of view. Chemostat cultures of this strain grown on glucose alone produced only acids (acetate, butyrate and lactate) and a high level of hydrogen. In contrast, when glycerol was metabolized in chemostat culture, 1,3-propanediol became the major product, the specific rate of acid formation decreased and a very low level of hydrogen was observed. In a fed-batch culture, the DG1(pSPD5) strain was able to produce 1,3-propanediol at a higher concentration (1104 mM) and productivity than the natural producer C. butyricum VPI 3266. Furthermore, this strain was also successfully used for very long term continuous production of 1,3-propanediol at high volumetric productivity (3 g L-1 h-1) and titer (788 mM).
María González-Pajuelo; Isabelle Meynial-Salles; Filipa Mendes; Jose Carlos Andrade; Isabel Vasconcelos; Philippe Soucaille
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-08-10
Journal Detail:
Title:  Metabolic engineering     Volume:  7     ISSN:  1096-7176     ISO Abbreviation:  Metab. Eng.     Publication Date:    2005 Sep-Nov
Date Detail:
Created Date:  2005-12-19     Completed Date:  2006-02-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9815657     Medline TA:  Metab Eng     Country:  Belgium    
Other Details:
Languages:  eng     Pagination:  329-36     Citation Subset:  IM    
Escola Superior Biotecnologia, Universidade Catolica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal.
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MeSH Terms
Cell Culture Techniques / methods
Clostridium acetobutylicum / genetics*,  metabolism*
Escherichia coli / genetics*,  metabolism
Genetic Enhancement / methods*
Glycerol / metabolism*
Industrial Microbiology / methods*
Propylene Glycols / metabolism*
Reg. No./Substance:
0/Propylene Glycols; 504-63-2/1,3-propanediol; 56-81-5/Glycerol

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