| Metabolic engineering of Arabidopsis and Brassica for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer production. | |
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MedLine Citation:
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PMID: 10504704 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Poly(hydroxyalkanoates) are natural polymers with thermoplastic properties. One polymer of this class with commercial applicability, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) can be produced by bacterial fermentation, but the process is not economically competitive with polymer production from petrochemicals. Poly(hydroxyalkanoate) production in green plants promises much lower costs, but producing copolymer with the appropriate monomer composition is problematic. In this study, we have engineered Arabidopsis and Brassica to produce PHBV in leaves and seeds, respectively, by redirecting the metabolic flow of intermediates from fatty acid and amino acid biosynthesis. We present a pathway for the biosynthesis of PHBV in plant plastids, and also report copolymer production, metabolic intermediate analyses, and pathway dynamics. |
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Authors:
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S Slater; T A Mitsky; K L Houmiel; M Hao; S E Reiser; N B Taylor; M Tran; H E Valentin; D J Rodriguez; D A Stone; S R Padgette; G Kishore; K J Gruys |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nature biotechnology Volume: 17 ISSN: 1087-0156 ISO Abbreviation: Nat. Biotechnol. Publication Date: 1999 Oct |
Date Detail:
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Created Date: 1999-11-02 Completed Date: 1999-11-02 Revised Date: 2005-10-14 |
Medline Journal Info:
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Nlm Unique ID: 9604648 Medline TA: Nat Biotechnol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1011-6 Citation Subset: IM |
Affiliation:
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Cereon Genomics, 45 Sidney St., Cambridge, MA 02139, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acyl Coenzyme A
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biosynthesis Amination Arabidopsis / metabolism* Brassica / metabolism* Butyric Acids / metabolism Magnetic Resonance Spectroscopy Polyesters / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Acyl Coenzyme A; 0/Butyric Acids; 0/Polyesters; 0/poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); 317-66-8/propionyl-coenzyme A; 600-18-0/alpha-ketobutyric acid |
| Comments/Corrections | |
Comment In:
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Nat Biotechnol. 1999 Oct;17(10):960-1
[PMID:
10504694
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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