Document Detail


Metabolic effects of static magnetic fields on Streptococcus pyogenes.
MedLine Citation:
PMID:  17486599     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study aimed to develop a simple experimental system utilising bacterial cells to investigate the dose responses resulting from exposures to static magnetic flux densities ranging from 0.05 to 0.5 T on viability, bacterial metabolism and levels of DNA damage in Streptococcus pyogenes. Exposure of S. pyogenes to a field of 0.3 T at 24 degrees C under anaerobic conditions resulted in a significant (P < 0.05) decrease in growth rate, with an increased mean generation time of 199 +/- 6 min compared to the control cells at 165 +/- 6 min (P < 0.05). Conversely, exposure to magnetic fields of 0.5 T significantly accelerated the growth rate at 24 degrees C compared to control cells, with a decreased mean generation time of 147 +/- 4 min (P < 0.05). The patterns of metabolite release from cells incubated in phosphate buffered saline (PBS) at 24 degrees C and exposed to different magnetic flux densities (0.05-0.5 T) were significantly (P < 0.05) altered, compared to non-exposed controls. Concentrations of metabolites, with the exception of aspartic acid (r = 0.44), were not linearly correlated with magnetic flux density, with all other r < 0.20. Instead, "window" effects were observed, with 0.25-0.3 T eliciting the maximal release of the majority of metabolites, suggesting that magnetic fields of these strengths had significant impacts on metabolic homeostasis in S. pyogenes. The exposure of cells to 0.3 T was also found to significantly reduce the yield of 8-hydroxyguanine in extracted DNA compared to controls, suggesting some possible anti-oxidant protection to S. pyogenes at this field strength.
Authors:
A C Morrow; R H Dunstan; B V King; T K Roberts
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioelectromagnetics     Volume:  28     ISSN:  0197-8462     ISO Abbreviation:  Bioelectromagnetics     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-08-08     Completed Date:  2007-11-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008281     Medline TA:  Bioelectromagnetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  439-45     Citation Subset:  IM    
Affiliation:
School of Environmental and Life Sciences, The University of Newcastle, Callaghan, New South Wales, Australia.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / metabolism*
Cell Survival / drug effects
DNA Damage / physiology*
DNA, Bacterial / radiation effects*
Dose-Response Relationship, Radiation
Electricity
Electromagnetic Fields*
Magnetics*
Radiation Dosage
Streptococcus pyogenes / cytology,  physiology*,  radiation effects*
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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