Document Detail


Metabolic effects of photodynamically induced apoptosis in an erythroleukemic cell line. A (31)P NMR spectroscopic study of Victoria-Blue-BO-sensitized TF-1 cells.
MedLine Citation:
PMID:  10699957     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Victoria Blue BO (VB BO) is a new and promising photosensitizer currently being evaluated for photodynamic therapy (PDT). Its photochemical processes are mediated by oxygen radicals, but do not involve singlet oxygen. We used (31)P NMR spectroscopy of VB-BO sensitized TF-1 leukemic cells to gain further insight into the biochemical mechanisms underlying PDT-induced cell death. Sham-treatment experiments were performed to evaluate the effects of this photosensitizer in the absence of light irradiation. Significant metabolic differences were detected for TF-1 cells incubated with VB BO but not exposed to light, as compared with native cells (controls). These changes include reductions in phosphocreatine, UDP-hexose and phosphodiester levels (as percentage of total phosphate) and slightly reduced intracellular pH. Complete phosphocreatine depletion, significant acidification and concomitant inorganic-phosphate accumulation were observed for TF-1 cells irradiated after incubation with VB BO. Moreover, significant changes in phospholipid metabolites, i.e., accumulation of cytidine 5'-diphosphate choline and a decrease in phosphodiester levels, were observed for PDT-treated vs. sham-treated cells. Perturbations of phospholipid metabolism may be involved in programmed cell death, and the detection of a characteristic DNA ladder pattern by gel electrophoresis confirmed the existence of apoptosis in PDT-treated TF-1 cells.
Authors:
A Viola; N W Lutz; C Maroc; C Chabannon; M Julliard; P J Cozzone
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  85     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  2000 Mar 
Date Detail:
Created Date:  2000-03-22     Completed Date:  2000-03-22     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  733-9     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Wiley-Liss, Inc.
Affiliation:
Laboratoire AM3, ESA-CNRS 6009, Faculté Saint-Jérôme, Marseille, France.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects*
Cell Survival / drug effects
Free Radicals / analysis
Humans
Hydrogen-Ion Concentration
Leukemia, Erythroblastic, Acute
Magnetic Resonance Spectroscopy
Phosphates / metabolism
Phosphatidylcholines / metabolism
Phosphatidylethanolamines / metabolism
Phosphocreatine / metabolism
Photochemotherapy
Photosensitizing Agents / toxicity*
Quaternary Ammonium Compounds / toxicity*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Free Radicals; 0/Phosphates; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Photosensitizing Agents; 0/Quaternary Ammonium Compounds; 2390-60-5/Victoria blue BO; 67-07-2/Phosphocreatine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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