Document Detail

Metabolic effects of glyphosate change the capacity of maize culture to regenerate plants.
MedLine Citation:
PMID:  19110340     Owner:  NLM     Status:  MEDLINE    
Since the presence of glyphosate in maize tissue cultures of proprietary line L2 was very detrimental to plant regeneration, we determined metabolic changes associated with the glyphosate effects on plant regeneration in maize cultures. The polar fraction composition and soluble and cell-wall-bound phenolics were analyzed in the regenerable (R) and non-regenerable (NR) calluses of maize line L2. The tissues with high regeneration capacity had low sugar and 4-aminobutyric acid (GABA) concentrations and increased concentrations of most amino acids, polyamines and indole-3-butenol in the soluble polar fraction and higher ferulic acid/coumaric acid and ferulic acid/diferulic acid ratios and higher levels of the predominant G (guaiacyl) units in the cell wall fraction compared with NR calluses as was found before with H99 and HiII maize R and NR tissues, indicating an association of these metabolites with the capacity of maize cultured tissue to regenerate plants. We also found that di-coumaroyl spermidine and coumaroyl-feruoyl spermidine are present in the soluble fraction of L2 R tissues and are practically absent in NR tissues. However, we did not see such differences in HiII and H99 samples, which indicate that these are genotypic features not related to the capacity to regenerate plants in maize tissue cultures. Glyphosate treatment caused the accumulation of shikimic and quinic acids (not detected in untreated samples) in R and NR calluses (with higher levels found in R tissues) and also decreased the FA/diFA ratio in cell wall phenolics, polyamine and amino acid levels, and increased sugar concentrations in the R L2 tissues, indicating a metabolic shift of R callus to NR tissues.
Alexander Ulanov; Anatoliy Lygin; David Duncan; Jack Widholm; Vera Lozovaya
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-12-24
Journal Detail:
Title:  Journal of plant physiology     Volume:  166     ISSN:  1618-1328     ISO Abbreviation:  J. Plant Physiol.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-11     Completed Date:  2009-07-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882059     Medline TA:  J Plant Physiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  978-87     Citation Subset:  IM    
Department of Crop Sciences, University of Illinois, 1201 W. Gregory Drive, Urbana, IL 61801, USA.
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MeSH Terms
Carbohydrate Metabolism / drug effects
Coumaric Acids / metabolism
Gene Expression Regulation, Plant / drug effects
Glycine / analogs & derivatives*,  pharmacology
Quinic Acid / metabolism
Regeneration / drug effects
Shikimic Acid / metabolism
Tissue Culture Techniques
Zea mays / drug effects,  metabolism*
gamma-Aminobutyric Acid / metabolism
Reg. No./Substance:
0/Coumaric Acids; 0/diferulic acid; 1071-83-6/glyphosate; 1135-24-6/ferulic acid; 138-59-0/Shikimic Acid; 56-12-2/gamma-Aminobutyric Acid; 56-40-6/Glycine; 77-95-2/Quinic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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