Document Detail

Metabolic development in the liver and the implications of the n-3 fatty acid supply.
MedLine Citation:
PMID:  22094600     Owner:  NLM     Status:  MEDLINE    
The n-3 fatty acids contribute to regulation of hepatic fatty acid oxidation and synthesis in adults and accumulate in fetal and infant liver in variable amounts depending on the maternal diet fat composition. Using 2D gel proteomics and matrix-assisted laser desorption/ionization time of flight mass spectrometry, we recently identified altered abundance of proteins associated with glucose and amino acid metabolism in neonatal rat liver with increased n-3 fatty acids. Here, we extend studies on n-3 fatty acids in hepatic metabolic development to targeted gene and metabolite analyses and map the results into metabolic pathways to consider the role of n-3 fatty acids in glucose, fatty acid, and amino metabolism. Feeding rats 1.5% compared with <0.1% energy 18:3n-3 during gestation led to higher 20:5n-3 and 22:6n-3 in 3-day-old offspring liver, higher serine hydroxymethyltransferase, carnitine palmitoyl transferase, and acyl CoA oxidase and lower pyruvate kinase and stearoyl CoA desaturase gene expression, with higher cholesterol, NADPH and glutathione, and lower glycine (P < 0.05). Integration of the results suggests that the n-3 fatty acids may be important in facilitating hepatic metabolic adaptation from in utero nutrition to the postnatal high-fat milk diet, by increasing fatty acid oxidation and directing glucose and amino acids to anabolic pathways.
Elizabeth M Novak; Bernd O Keller; Sheila M Innis
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-11-17
Journal Detail:
Title:  American journal of physiology. Gastrointestinal and liver physiology     Volume:  302     ISSN:  1522-1547     ISO Abbreviation:  Am. J. Physiol. Gastrointest. Liver Physiol.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-01-04     Completed Date:  2012-02-16     Revised Date:  2012-05-23    
Medline Journal Info:
Nlm Unique ID:  100901227     Medline TA:  Am J Physiol Gastrointest Liver Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  G250-9     Citation Subset:  IM    
Department of Pediatrics, Nutrition and Metabolism Program, Child and Family Research Institute, University of British Columbia, Vancouver, British Columbia, Canada.
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MeSH Terms
Acyl-CoA Oxidase / genetics,  metabolism
Carnitine O-Palmitoyltransferase / genetics,  metabolism
Cholesterol / metabolism
Fatty Acids, Omega-3 / administration & dosage*
Gene Expression
Glutathione / genetics,  metabolism
Glycine / genetics,  metabolism
Glycine Hydroxymethyltransferase / genetics,  metabolism
Lipid Metabolism
Liver / enzymology*,  metabolism
NADP / genetics,  metabolism
Pyruvate Kinase / genetics,  metabolism
Stearoyl-CoA Desaturase / genetics,  metabolism
Grant Support
//Canadian Institutes of Health Research
Reg. No./Substance:
0/Fatty Acids, Omega-3; 53-59-8/NADP; 56-40-6/Glycine; 57-88-5/Cholesterol; 70-18-8/Glutathione; EC Desaturase; EC Oxidase; EC Hydroxymethyltransferase; EC O-Palmitoyltransferase; EC Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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