Document Detail


Metabolic cycling without cell division cycling in respiring yeast.
MedLine Citation:
PMID:  22065748     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Despite rapid progress in characterizing the yeast metabolic cycle, its connection to the cell division cycle (CDC) has remained unclear. We discovered that a prototrophic batch culture of budding yeast, growing in a phosphate-limited ethanol medium, synchronizes spontaneously and goes through multiple metabolic cycles, whereas the fraction of cells in the G1/G0 phase of the CDC increases monotonically from 90 to 99%. This demonstrates that metabolic cycling does not require cell division cycling and that metabolic synchrony does not require carbon-source limitation. More than 3,000 genes, including most genes annotated to the CDC, were expressed periodically in our batch culture, albeit a mere 10% of the cells divided asynchronously; only a smaller subset of CDC genes correlated with cell division. These results suggest that the yeast metabolic cycle reflects a growth cycle during G1/G0 and explains our previous puzzling observation that genes annotated to the CDC increase in expression at slow growth.
Authors:
Nikolai Slavov; Joanna Macinskas; Amy Caudy; David Botstein
Related Documents :
2089108 - Human caki-1 cells are the first model for extraneuronal transport of noradrenaline (up...
12970388 - Cellular uptake and efflux of the tea flavonoid (-)epicatechin-3-gallate in the human i...
19303398 - Betapix and git1 regulate hgf-induced lamellipodia formation and wave2 transport.
7263768 - Hexose sugar transport activity in a mouse fibroblast cell line temperature sensitive f...
25123148 - Ultrasensitive detection of cancer cells and glycan expression profiling based on a mul...
8600348 - Induced mitotic death of hela cells by abnormal expression of c-h-ras.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-11-07
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  108     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-23     Completed Date:  2012-01-27     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  19090-5     Citation Subset:  IM    
Affiliation:
Departments of Biology and Physics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. nslavov@alum.mit.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cell Division / physiology*
Gene Expression Profiling
Gene Expression Regulation, Fungal / physiology*
Genes, Fungal / genetics*
Metabolic Networks and Pathways / physiology*
Microarray Analysis
Oxygen Consumption / physiology
RNA, Messenger / analysis
Saccharomycetales / growth & development,  metabolism*
Grant Support
ID/Acronym/Agency:
GM046406/GM/NIGMS NIH HHS; GM071508/GM/NIGMS NIH HHS; R01 GM046406/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Messenger
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Conserved metabolic energy production pathways govern Eiger/TNF-induced nonapoptotic cell death.
Next Document:  Genomic dissection of the epidermal growth factor receptor (EGFR)/PI3K pathway reveals frequent dele...