Document Detail


Metabolic cycling without cell division cycling in respiring yeast.
MedLine Citation:
PMID:  22065748     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Despite rapid progress in characterizing the yeast metabolic cycle, its connection to the cell division cycle (CDC) has remained unclear. We discovered that a prototrophic batch culture of budding yeast, growing in a phosphate-limited ethanol medium, synchronizes spontaneously and goes through multiple metabolic cycles, whereas the fraction of cells in the G1/G0 phase of the CDC increases monotonically from 90 to 99%. This demonstrates that metabolic cycling does not require cell division cycling and that metabolic synchrony does not require carbon-source limitation. More than 3,000 genes, including most genes annotated to the CDC, were expressed periodically in our batch culture, albeit a mere 10% of the cells divided asynchronously; only a smaller subset of CDC genes correlated with cell division. These results suggest that the yeast metabolic cycle reflects a growth cycle during G1/G0 and explains our previous puzzling observation that genes annotated to the CDC increase in expression at slow growth.
Authors:
Nikolai Slavov; Joanna Macinskas; Amy Caudy; David Botstein
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-11-07
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  108     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-23     Completed Date:  2012-01-27     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  19090-5     Citation Subset:  IM    
Affiliation:
Departments of Biology and Physics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. nslavov@alum.mit.edu
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MeSH Terms
Descriptor/Qualifier:
Cell Division / physiology*
Gene Expression Profiling
Gene Expression Regulation, Fungal / physiology*
Genes, Fungal / genetics*
Metabolic Networks and Pathways / physiology*
Microarray Analysis
Oxygen Consumption / physiology
RNA, Messenger / analysis
Saccharomycetales / growth & development,  metabolism*
Grant Support
ID/Acronym/Agency:
GM046406/GM/NIGMS NIH HHS; GM071508/GM/NIGMS NIH HHS; R01 GM046406/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Messenger
Comments/Corrections

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