| Metabolic cycling without cell division cycling in respiring yeast. | |
| | |
MedLine Citation:
|
PMID: 22065748 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Despite rapid progress in characterizing the yeast metabolic cycle, its connection to the cell division cycle (CDC) has remained unclear. We discovered that a prototrophic batch culture of budding yeast, growing in a phosphate-limited ethanol medium, synchronizes spontaneously and goes through multiple metabolic cycles, whereas the fraction of cells in the G1/G0 phase of the CDC increases monotonically from 90 to 99%. This demonstrates that metabolic cycling does not require cell division cycling and that metabolic synchrony does not require carbon-source limitation. More than 3,000 genes, including most genes annotated to the CDC, were expressed periodically in our batch culture, albeit a mere 10% of the cells divided asynchronously; only a smaller subset of CDC genes correlated with cell division. These results suggest that the yeast metabolic cycle reflects a growth cycle during G1/G0 and explains our previous puzzling observation that genes annotated to the CDC increase in expression at slow growth. |
| | |
Authors:
|
Nikolai Slavov; Joanna Macinskas; Amy Caudy; David Botstein |
Related Documents
:
|
2909368 - Triiodothyronine transport into differentiated and undifferentiated mouse neuroblastoma... 22749998 - Eicosapentaenoic acid attenuates statin-induced er stress and toxicity in myoblast. 8086488 - The transporter for the hmg-coa reductase inhibitor pravastatin is not present in hep g... 6097528 - Ion-transporting atpases and matrix mineralization in cultured osteoblastlike cells. 1170948 - Matrix simulation of duodenal crypt cell kinetics. ii. cell kinetics following hydroxyu... 3406808 - Survival of vero, myeloma and hybridoma cells during cold storage. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-11-07 |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 108 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2011-11-23 Completed Date: 2012-01-27 Revised Date: 2012-10-09 |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
|
Languages: eng Pagination: 19090-5 Citation Subset: IM |
Affiliation:
|
Departments of Biology and Physics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. nslavov@alum.mit.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Division
/
physiology* Gene Expression Profiling Gene Expression Regulation, Fungal / physiology* Genes, Fungal / genetics* Metabolic Networks and Pathways / physiology* Microarray Analysis Oxygen Consumption / physiology RNA, Messenger / analysis Saccharomycetales / growth & development, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
GM046406/GM/NIGMS NIH HHS; GM071508/GM/NIGMS NIH HHS; R01 GM046406/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Conserved metabolic energy production pathways govern Eiger/TNF-induced nonapoptotic cell death.
Next Document: Genomic dissection of the epidermal growth factor receptor (EGFR)/PI3K pathway reveals frequent dele...