| Metabolic control analysis of cellular respiration in situ in intraoperational samples of human breast cancer. | |
| | |
MedLine Citation:
|
PMID: 22836527 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The aim of this study was to analyze quantitatively cellular respiration in intraoperational tissue samples taken from human breast cancer (BC) patients. We used oxygraphy and the permeabilized cell techniques in combination with Metabolic Control Analysis (MCA) to measure a corresponding flux control coefficient (FCC). The activity of all components of ATP synthasome, and respiratory chain complexes was found to be significantly increased in human BC cells in situ as compared to the adjacent control tissue. FCC(s) were determined upon direct activation of respiration with exogenously-added ADP and by titrating the complexes with their specific inhibitors to stepwise decrease their activity. MCA showed very high sensitivity of all complexes and carriers studied in human BC cells to inhibition as compared to mitochondria in normal oxidative tissues. The sum of FCC(s) for all ATP synthasome and respiratory chain components was found to be around 4, and the value exceeded significantly that for normal tissue (close to 1). In BC cells, the key sites of the regulation of respiration are Complex IV (FCC = 0.74), ATP synthase (FCC = 0.61), and phosphate carrier (FCC = 0.60); these FCC(s) exceed considerably (~10-fold) those for normal oxidative tissues. In human BC cells, the outer mitochondrial membrane is characterized by an increased permeability towards adenine nucleotides, the mean value of the apparent K(m) for ADP being equal to 114.8 ± 13.6 μM. Our data support the two-compartment hypothesis of tumor metabolism, the high sum of FCC(s) showing structural and functional organization of mitochondrial respiratory chain and ATP synthasome as supercomplexes in human BC. |
| | |
Authors:
|
Tuuli Kaambre; Vladimir Chekulayev; Igor Shevchuk; Minna Karu-Varikmaa; Natalja Timohhina; Kersti Tepp; Jelena Bogovskaja; Riina Kütner; Vahur Valvere; Valdur Saks |
Related Documents
:
|
22287017 - The effect of hyperosmolar stimuli and cyclophosphamide on the culture of normal rat ur... 6274137 - Malignant fibrous histiocytoma of bone associated with focal hemangiopericytomatous pat... 3103357 - Distribution pattern of concanavalin a on carcinoma cells, histiocytes and mesothelial ... 21295777 - Differential effects of a complex organochlorine mixture on the proliferation of breast... 22221567 - Review of experimental animal models of biliary acute pancreatitis and recent advances ... 19833387 - Metabolism of tegdma and hema in human cells. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-7-27 |
Journal Detail:
|
Title: Journal of bioenergetics and biomembranes Volume: - ISSN: 1573-6881 ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
|
Created Date: 2012-7-27 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7701859 Medline TA: J Bioenerg Biomembr Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Laboratory of Bioenergetics, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, Tallinn, Estonia, tuuli.kaambre@kbfi.ee. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Rotator cuff muscles perform different functional roles during shoulder external rotation exercises.
Next Document: Where is obesity prevention on the map?: distribution and predictors of local health department pre...