Document Detail


Metabolic characteristics of human subcutaneous abdominal adipose tissue after overnight fast.
MedLine Citation:
PMID:  22167523     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Subcutaneous abdominal adipose tissue is one of the largest fat depots and contributes the major proportion of circulating nonesterified fatty acids (NEFA). Little is known about aspects of human adipose tissue metabolism in vivo other than lipolysis. Here we collated data from 331 experiments in 255 healthy volunteers over a 23-year period, in which subcutaneous abdominal adipose tissue metabolism was studied by measurements of arterio-venous differences after an overnight fast. NEFA and glycerol were released in a ratio of 2.7:1, different (P < 0.001) from the value of 3.0 that would indicate no fatty acid re-esterification. Fatty acid re-esterification was 10.2 ± 1.4%. Extraction of triacylglycerol (TG) (fractional extraction 5.7 ± 0.4%) indicated intravascular lipolysis by lipoprotein lipase, and this contributed 21 ± 3% of the glycerol released. Glucose uptake (fractional extraction 2.6 ± 0.3%) was partitioned around 20-25% for provision of glycerol 3-phosphate and 30% into lactate production. There was release of lactate and pyruvate, with extraction of the ketone bodies 3-hydroxybutyrate and acetoacetate, although these were small numerically compared with TG and glucose uptake. NEFA release (expressed per 100 g tissue) correlated inversely with measures of fat mass (e.g., with BMI, r(s) = -0.24, P < 0.001). We examined within-person variability. Systemic NEFA concentrations, NEFA release, fatty acid re-esterification, and adipose tissue blood flow were all more consistent within than between individuals. This picture of human adipose tissue metabolism in the fasted state should contribute to a greater understanding of adipose tissue physiology and pathophysiology.
Authors:
Keith N Frayn; Sandy M Humphreys
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Publication Detail:
Type:  Journal Article; Meta-Analysis; Research Support, Non-U.S. Gov't     Date:  2011-12-13
Journal Detail:
Title:  American journal of physiology. Endocrinology and metabolism     Volume:  302     ISSN:  1522-1555     ISO Abbreviation:  Am. J. Physiol. Endocrinol. Metab.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-06     Completed Date:  2012-03-30     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  100901226     Medline TA:  Am J Physiol Endocrinol Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  E468-75     Citation Subset:  IM    
Affiliation:
Oxford Centre for Diabetes, Endocrinology & Metabolism, Churchill Hospital, Oxford OX3 7LJ, UK. keith.frayn@ocdem.ox.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Blood Glucose / metabolism
Body Mass Index
Fasting / metabolism*
Fatty Acids, Nonesterified / blood,  metabolism
Female
Glycerol / blood,  metabolism
Glycerophosphates / metabolism
Humans
Insulin / blood,  metabolism
Ketone Bodies / metabolism
Lactates / metabolism
Lipolysis
Lipoprotein Lipase / metabolism
Male
Pyruvates / metabolism
Subcutaneous Fat, Abdominal / metabolism*
Triglycerides / blood,  metabolism
Grant Support
ID/Acronym/Agency:
//British Heart Foundation; //Medical Research Council; //Wellcome Trust
Chemical
Reg. No./Substance:
0/Blood Glucose; 0/Fatty Acids, Nonesterified; 0/Glycerophosphates; 0/Insulin; 0/Ketone Bodies; 0/Lactates; 0/Pyruvates; 0/Triglycerides; 56-81-5/Glycerol; 9NTI6P3O4X/alpha-glycerophosphoric acid; EC 3.1.1.34/Lipoprotein Lipase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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