Document Detail

Metabolic characteristics of different malignant cancer cell lines.
MedLine Citation:
PMID:  9858895     Owner:  NLM     Status:  MEDLINE    
Extracellular AMP inhibits cell proliferation of MCF-7 and MDA-MB-453 whereas cell proliferation of the highly malignant Novikoff cell line is not affected. In medium with low glucose supply MDA-MB-453 cells grow well, Novikoff cells are slightly inhibited and MCF-7 cells are totally unable to grow. Isoelectric focusing revealed that a glyclytic enzyme complex exists in all three cell lines. In addition to the glycolytic enzymes, c-Raf-kinase, adenylate kinase, and nucleoside diphosphate kinase are also found within the complex. The differences in glucose in dependence of the three cell lines can be explained by the different constitutions of shuttle enzymes. MDA-MB-453 and Novikoff cells contain cytsolic glycerol 3-phosphate dehydrogenase which is associated with glyceraldehyde 3-phosphate dehydrogenase within the glycolytic enzyme complex and which is responsible for the transport of cytoslic hydrogen in the mitochondria. MCF-7 and Novikoff cells contain the pI 7.8 form of malate dehydrogenase which couples glycolysis with glutaminolysis.
S Mazurek; H Grimm; S Wilker; S Leib; E Eigenbrodt
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Anticancer research     Volume:  18     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    1998 Sep-Oct
Date Detail:
Created Date:  1998-12-29     Completed Date:  1998-12-29     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  GREECE    
Other Details:
Languages:  eng     Pagination:  3275-82     Citation Subset:  IM    
Institute of Biochemistry and Endocrinology, Veterinary Faculty, University of Giessen, Germany.
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MeSH Terms
Adenylate Kinase / metabolism
Aspartic Acid / metabolism
Breast Neoplasms / enzymology,  metabolism
Glucose / metabolism*
Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism
Liver Neoplasms, Experimental / enzymology,  metabolism
NAD / metabolism
Neoplasms / enzymology,  metabolism*
Nucleoside-Diphosphate Kinase / metabolism
Phosphoglycerate Kinase / metabolism
Phosphoglycerate Mutase / metabolism
Phosphopyruvate Hydratase / metabolism
Pyruvate Kinase / metabolism
Tumor Cells, Cultured / enzymology,  metabolism
Reg. No./Substance:
50-99-7/Glucose; 53-84-9/NAD; 56-84-8/Aspartic Acid; EC 1.2.1.-/Glyceraldehyde-3-Phosphate Dehydrogenases; EC Kinase; EC Kinase; EC Kinase; EC Kinase; EC Hydratase; EC Mutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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