| Metabolic changes in a pyruvate kinase gene deletion mutant of Corynebacterium glutamicum ATCC 13032. | |
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MedLine Citation:
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PMID: 20144730 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To investigate primary effects of a pyruvate kinase (PYK) defect on glucose metabolism in Corynebacterium glutamicum, a pyk-deleted mutant was derived from wild-type C. glutamicum ATCC13032 using the double-crossover chromosome replacement technique. The mutant was then evaluated under glutamic acid-producing conditions induced by biotin limitation. The mutant showed an increased specific rate of glucose consumption, decreased growth, higher glutamic acid production, and aspartic acid formation during the glutamic acid production phase. A significant increase in phosphoenolpyruvate (PEP) carboxylase activity and a significant decrease in PEP carboxykinase activity occurred in the mutant, which suggested an enhanced overall flux of the anaplerotic pathway from PEP to oxaloacetic acid in the mutant. The enhanced anaplerotic flux may explain both the increased rate of glucose consumption and the higher productivity of glutamic acid in the mutant. Since the pyk-complemented strain had similar metabolic profiles to the wild-type strain, the observed changes represented intrinsic effects of pyk deletion on the physiology of C. glutamicum. |
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Authors:
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Kazunori Sawada; Susumu Zen-in; Masaru Wada; Atsushi Yokota |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-06 |
Journal Detail:
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Title: Metabolic engineering Volume: 12 ISSN: 1096-7184 ISO Abbreviation: Metab. Eng. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-08-04 Completed Date: 2010-11-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9815657 Medline TA: Metab Eng Country: Belgium |
Other Details:
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Languages: eng Pagination: 401-7 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University, Kita-ku, Sapporo, Hokkaido 060-8589, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aspartic Acid
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biosynthesis Biotin / metabolism Corynebacterium glutamicum / enzymology*, genetics* Glucose / metabolism Glutamic Acid / biosynthesis Metabolic Networks and Pathways* Oxaloacetate / analysis Phosphoenolpyruvate Carboxykinase (ATP) / analysis Phosphoenolpyruvate Carboxylase / analysis Pyruvate Kinase / genetics*, metabolism* Sequence Deletion |
| Chemical | |
Reg. No./Substance:
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0/Oxaloacetate; 50-99-7/Glucose; 56-84-8/Aspartic Acid; 56-86-0/Glutamic Acid; 58-85-5/Biotin; EC 2.7.1.40/Pyruvate Kinase; EC 4.1.1.31/Phosphoenolpyruvate Carboxylase; EC 4.1.1.49/Phosphoenolpyruvate Carboxykinase (ATP) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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