Document Detail


Metabolic biomarkers for monitoring anaerobic naphthalene biodegradation in situ.
MedLine Citation:
PMID:  12220410     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During the anaerobic biodegradation of naphthalene and methylnaphthalene, unique metabolites are formed by specific microbial carboxylation and ring-reduction reactions. Groundwater samples from an anoxic, shallow aquifer contaminated with gasoline were examined for the presence of these metabolites by extraction, derivatization and gas chromatography coupled to mass spectroscopy. Several metabolites [2-naphthoic acid (2-NA), tetrahydro-2-naphthoic acid (TH-2-NA), hexahydro-2-naphthoic acid (HH-2-NA) and methylnaphthoic acid (MNA)] were found to be present in the groundwater samples. The concentration of 2-NA at each monitoring well was quantified and correlated to the zones of contamination. The presence of the other metabolites in the same wells as 2-NA was used as confirmation that the anaerobic pathway was indeed active. The distribution of metabolites at this site shows that they can be used as biomarkers for demonstrating in situ biodegradation.
Authors:
Craig D Phelps; Joseph Battistelli; L Y Young
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Environmental microbiology     Volume:  4     ISSN:  1462-2912     ISO Abbreviation:  Environ. Microbiol.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-09-10     Completed Date:  2002-10-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100883692     Medline TA:  Environ Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  532-7     Citation Subset:  IM    
Affiliation:
Biotechnology Center for Agriculture and the Environment, Cook College, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA. phelps@envsci.rutgers.edu
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MeSH Terms
Descriptor/Qualifier:
Bacteria, Anaerobic / metabolism*
Biodegradation, Environmental
Biological Markers / analysis
Gas Chromatography-Mass Spectrometry
Naphthalenes / metabolism*
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Naphthalenes; 91-20-3/naphthalene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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