| Metabolic analysis of body fluids by capillary electrophoresis using noncovalently coated capillaries. | |
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MedLine Citation:
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PMID: 18565806 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The potential of capillaries noncovalently coated with charged polymers for the metabolic analysis of body fluids by CE is illustrated. Firstly, the usefulness of a coating consisting of a triple layer of polybrene-dextran sulfate-polybrene for the fast analysis of organic acids is described. The CE system allowed direct injections of CSF, plasma and urine samples, yielding good separation efficiencies. RSDs for migration times and peak areas of organic acids in plasma were <3% and <5%, respectively. The usefulness of the system is illustrated by the profiling of organic acids in plasma and urine samples. Secondly, a CE system comprising a bilayer coating of polybrene-poly(vinylsulfonate), which provides a considerable EOF at low pH is described. This system was combined with TOF-MS and used for the fast analysis of amino acids in cerebrospinal fluid (CSF) and urine with minimal sample pretreatment. RSDs for migration times and peak areas of amino acids in CSF and urine were <2% and <10%, respectively. The applicability of the system is demonstrated by the profiling of endogenous low-molecular weight metabolites in CSF from a healthy individual and a patient with complex regional pain syndrome. |
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Authors:
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Rawi Ramautar; Oleg A Mayboroda; André M Deelder; Govert W Somsen; Gerhardus J de Jong |
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Publication Detail:
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Type: Journal Article Date: 2008-06-06 |
Journal Detail:
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Title: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Volume: 871 ISSN: 1570-0232 ISO Abbreviation: J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-08-12 Completed Date: 2008-10-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101139554 Medline TA: J Chromatogr B Analyt Technol Biomed Life Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 370-4 Citation Subset: IM |
Affiliation:
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Department of Biomedical Analysis, Utrecht University, P.O. Box 80082, 3508 TB Utrecht, The Netherlands. R.Ramautar@uu.nl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Body Fluids
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metabolism* Chromatography, Micellar Electrokinetic Capillary / methods* Electrophoresis, Capillary / instrumentation*, methods* Humans Mass Spectrometry / methods* Reproducibility of Results |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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