| Metabolic alternations in SOD-deficient Escherichia coli cells when cultivated under oxidative stress from photoexcited titanium dioxide. | |
| | |
MedLine Citation:
|
PMID: 18338221 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Superoxide dismutase (SOD)-deficient Escherichia coli was cultivated under the oxidative stress generated by photoexcited titanium dioxide. These cells showed higher growth rate and glucose consumption rate with accelerated accumulation of acetic acid in the medium, compared to the cells cultivated under the normal condition without the stress. Under the stress condition, the activity of acetate kinase and mRNA expressions of the enzymes for acetic acid production (pta and ackA) were approximately doubled, while the activity of citrate synthase and mRNA expressions of the enzymes in TCA cycle (gltA, acnA, icd, sucA, sucC, sdhA, fumA and mdh) were repressed by about half, as compared with those under the normal condition. These results suggest that the stress-suffering cells switch the metabolic pathway into a "suppressed aerobiosis", possibly for lowering the generation of reactive oxygen species. |
| | |
Authors:
|
Yoshihiro Ojima; Motomu Nishioka; Masahito Taya |
Publication Detail:
|
Type: Journal Article Date: 2008-03-13 |
Journal Detail:
|
Title: Biotechnology letters Volume: 30 ISSN: 0141-5492 ISO Abbreviation: Biotechnol. Lett. Publication Date: 2008 Jun |
Date Detail:
|
Created Date: 2008-04-22 Completed Date: 2008-07-01 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 1107-13 Citation Subset: IM |
Affiliation:
|
Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka, 560-8531, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetate Kinase
/
metabolism Acetic Acid / metabolism Citrate (si)-Synthase / metabolism Citric Acid Cycle Escherichia coli / enzymology, metabolism* Glucose / metabolism Oxidative Stress* Reactive Oxygen Species / metabolism Superoxide Dismutase / metabolism* Titanium / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Reactive Oxygen Species; 13463-67-7/titanium dioxide; 50-99-7/Glucose; 64-19-7/Acetic Acid; 7440-32-6/Titanium; EC 1.15.1.1/Superoxide Dismutase; EC 2.3.3.1/Citrate (si)-Synthase; EC 2.7.2.1/Acetate Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Correlation between cell morphology and aggrecan gene expression level during differentiation from m...
Next Document: Visual exploration of structure-activity relationship using maximum common framework.