| Metabolic alterations in organic acids and gamma-aminobutyric acid in developing tomato (Solanum lycopersicum L.) fruits. | |
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MedLine Citation:
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PMID: 20595461 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Salt stress improves the quality of tomato fruits. To clarify the mechanism(s) underlying this phenomenon, we investigated metabolic alterations in tomato fruits exposed to 160 mM salt, focusing on metabolism of organic acids related to the tricarboxylic acid (TCA) cycle and gamma-aminobutyric acid (GABA). Quantitative analyses revealed that most amino acids increased in response to salt stress throughout fruit development, and the effect of the stress was greater in the pericarp than in the columella, whereas organic acids did not show a remarkable tendency to salt stress. The transcript levels of 20 genes encoding enzymes of the TCA cycle and peripheral pathways were also analyzed in salt-stressed fruit. Genes responsive to salt stress could be categorized into two types, which were expressed during early development or ripening stages. During fruit development, phosphoenolpyruvate carboxylase 2 and phosphoenolpyruvate carboxykinase displayed contrasting expression patterns between early development and ripening, suggesting a switch of carbohydrate metabolism after the turning stage. Our results revealed a new metabolic pathway for GABA during the development of tomato fruits. At the start of ripening, GABA is first converted to malate via succinate semialdehyde, and it passes into a shunt through pyruvate. Then, it flows back to the TCA cycle and is stored as citrate, which contributes as a substrate for respiration during fruit maturation. |
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Authors:
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Yong-Gen Yin; Takehiro Tominaga; Yoko Iijima; Koh Aoki; Daisuke Shibata; Hiroshi Ashihara; Shigeo Nishimura; Hiroshi Ezura; Chiaki Matsukura |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-01 |
Journal Detail:
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Title: Plant & cell physiology Volume: 51 ISSN: 1471-9053 ISO Abbreviation: Plant Cell Physiol. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-13 Completed Date: 2010-11-23 Revised Date: 2010-12-15 |
Medline Journal Info:
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Nlm Unique ID: 9430925 Medline TA: Plant Cell Physiol Country: Japan |
Other Details:
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Languages: eng Pagination: 1300-14 Citation Subset: IM |
Affiliation:
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Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism Citric Acid Cycle* Fruit / chemistry*, growth & development Gene Expression Regulation, Plant Lycopersicon esculentum / chemistry, genetics, growth & development* RNA, Plant / genetics Sodium Chloride / metabolism Stress, Physiological gamma-Aminobutyric Acid / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/RNA, Plant; 56-12-2/gamma-Aminobutyric Acid; 7647-14-5/Sodium Chloride |
| Comments/Corrections | |
Erratum In:
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Plant Cell Physiol. 2010 Nov;51(11):1950 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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