Document Detail


Metabolic adaptations of liver mitochondria during restricted feeding schedules.
MedLine Citation:
PMID:  15976385     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Food anticipatory activity (FAA) is an output of the food-entrained oscillator (FEO), a conspicuous biological clock that expresses when experimental animals are under a restricted food schedule (RFS). We have shown that the liver is entrained by RFS and exhibits an anticipatory response before meal time in its oxidative and energetic state. The present study was designed to determine the mitochondrial oxidative and phosphorylating capacity in the liver of rats under RFS to further support the biochemical anticipatory role that this organ plays during the food entrainment (9). Metabolic and functional parameters of liver mitochondria were characterized before (0800 h), during (1100 h), and after (1400 h) FAA. The main results were as follows. First, there was an enhancement during FAA (1100 h) in 1) oxidative capacity (site I of the electron transport chain), 2) phosphorylating ability (estimated by ATP synthesis), and 3) activities of NADH shuttles. Second, after rats were fed (1400 h), the phosphorylating capacity remained high, but this was not the case for the respiratory control ratio for site I. Finally, in the three experimental conditions before, during, and after FAA, an increment was detected in the H(+) electrochemical potential, due to an elevation in mitochondrial membrane potential, and in mitochondrial yield. Most of the changes in mitochondrial properties related to RFS were also present when results were compared with those from the 24-h fasted group. In conclusion, the results support the notion that a distinctive rheostatic state is installed in the metabolic activity of the liver when FEO is being expressed.
Authors:
Adrián Báez-Ruiz; Carolina Escobar; Raúl Aguilar-Roblero; Olivia Vázquez-Martínez; Mauricio Díaz-Muñoz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-06-23
Journal Detail:
Title:  American journal of physiology. Gastrointestinal and liver physiology     Volume:  289     ISSN:  0193-1857     ISO Abbreviation:  Am. J. Physiol. Gastrointest. Liver Physiol.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-11-15     Completed Date:  2005-12-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100901227     Medline TA:  Am J Physiol Gastrointest Liver Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  G1015-23     Citation Subset:  IM    
Affiliation:
Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, Universidad Nacional Autónoma de México-Juriquilla, Querétaro, Qro 76001, México.
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MeSH Terms
Descriptor/Qualifier:
Animals
Citrate (si)-Synthase / metabolism
Conditioning, Operant
Fasting / metabolism
Food Deprivation / physiology*
Glutamic Acid / metabolism
Malates / metabolism
Male
Membrane Potentials
Mitochondria, Liver / metabolism*
Mitochondrial Swelling
Rats
Rats, Wistar
Succinic Acid / metabolism
Chemical
Reg. No./Substance:
0/Malates; 110-15-6/Succinic Acid; 56-86-0/Glutamic Acid; 6915-15-7/malic acid; EC 2.3.3.1/Citrate (si)-Synthase

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