Document Detail


Metabolic activation of bladder procarcinogens, 2-aminofluorene, 4-aminobiphenyl, and benzidine by Pseudomonas aeruginosa and other human endogenous bacteria.
MedLine Citation:
PMID:  16203120     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pseudomonas aeruginosa, an opportunistic pathogen of the human urinary tract, and other selected human endogenous bacteria were investigated for metabolic activation of the bladder procarcinogens, 2-aminofluorene (2-AF), 4-aminobiphenyl (4-AB), and benzidine (Bz). The cell-free extracts of Pseudomonas aeruginosa, Escherichia coli, Enterobacter aerogenes, Proteus mirabilis, Proteus vulgaris, Staphylococcus epidermidis, Staphylococcus saprophyticus, Klebsiella pneumoniae, and intestinal anaerobes, Bacteroides fragilis, Clostridium perfringens, and Eubacterium aerofaciens produced increased histidine revertant frequencies with the tester strain Salmonella typhimurium TA98 in the Ames Salmonella mutagenicity assay. In addition, the cell-free extracts of Pseudomonas aeruginosa, Bacteroides fragilis, and Eubacterium aerofaciens each showed the presence of a cytochrome P450 absorption peak in the carbon monoxide (CO) difference spectrum. This was not demonstratable for the other bacteria. Our findings indicate that human endogenous bacteria, which are opportunistic pathogens of the urinary bladder, can metabolically activate the bladder procarcinogens 2-AF, 4-AB, and Bz into mutagens. The metabolic activation by Pseudomonas aeruginosa, Bacteroides fragilis, and Eubacterium aerofaciens is mediated by a cytochrome P450 enzyme. For those organisms that induced metabolic activation but did not show a P450 absorption peak with the cell-free extracts, other oxidative enzymes may be involved.
Authors:
Piyatilake Adris; King-Thom Chung
Publication Detail:
Type:  In Vitro; Journal Article     Date:  2005-10-03
Journal Detail:
Title:  Toxicology in vitro : an international journal published in association with BIBRA     Volume:  20     ISSN:  0887-2333     ISO Abbreviation:  Toxicol In Vitro     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-03-10     Completed Date:  2006-06-07     Revised Date:  2009-04-10    
Medline Journal Info:
Nlm Unique ID:  8712158     Medline TA:  Toxicol In Vitro     Country:  England    
Other Details:
Languages:  eng     Pagination:  367-74     Citation Subset:  IM    
Affiliation:
Department of Biology, The University of Memphis, 3774 Walker Street, Memphis, TN 38152, USA.
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MeSH Terms
Descriptor/Qualifier:
Aminobiphenyl Compounds / metabolism*,  toxicity*
Animals
Bacteria / metabolism*
Benzidines / metabolism*,  toxicity*
Biotransformation*
Carbon Monoxide / chemistry,  metabolism
Carcinogens / metabolism*,  toxicity*
Cytochrome P-450 Enzyme System / metabolism
Fluorenes / metabolism*,  toxicity*
Humans
Isoenzymes / metabolism
Mutagenicity Tests
Pseudomonas aeruginosa / metabolism*
Rats
Salmonella typhimurium / drug effects,  genetics
Chemical
Reg. No./Substance:
0/Aminobiphenyl Compounds; 0/Benzidines; 0/Carcinogens; 0/Fluorenes; 0/Isoenzymes; 153-78-6/2-aminofluorene; 630-08-0/Carbon Monoxide; 9035-51-2/Cytochrome P-450 Enzyme System; 92-67-1/4-biphenylamine; 92-87-5/benzidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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