| Metabolic profiling of plasma in overweight/obese and lean men using ultra performance liquid chromatography and Q-TOF mass spectrometry (UPLC-Q-TOF MS). | |
| | |
MedLine Citation:
|
PMID: 20560578 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Obesity is currently epidemic in many countries worldwide and is strongly related to diabetes and cardiovascular disease. This study investigated the differences in metabolomic profiling between overweight/obese and normal-weight men. Overweight/obese (n=30) and age-matched, normal-weight men (n=30) were included. Anthropometric parameters, conventional metabolites, and biomarkers were measured. Metabolomic profiling was analyzed with UPLC-Q-TOF MS. Overweight/obese men showed higher levels of HOMA-IR, triglycerides, total cholesterol, and LDL-cholesterol, and lower levels of HDL-cholesterol and adiponectin than lean men. Overweight/obese men showed higher proportion of stearic acid and lower proportion of oleic acid in serum phospholipids. Additionally, overweight/obese individuals showed higher fat intake and lower ratio of polyunsaturated fatty acids to saturated fatty acids. We identified three lyso-phosphatidylcholine (lysoPC) as potential plasma markers and confirmed eight known metabolites for overweight/obesity men. Especially, overweight/obese subjects showed higher levels of lysoPC C14:0 and lysoPC C18:0 and lower levels of lysoPC C18:1 than lean subjects. Results confirmed abnormal metabolism of two branched-chain amino acids, two aromatic amino acids, and fatty acid synthesis and oxidation in overweight/obese men. Additionally, the amount of dietary saturated fat may influence the proportion of saturated fatty acids in serum phospholipids and the degree of saturation of the constituent acyl group of plasma lysoPC. |
| | |
Authors:
|
Ji Young Kim; Ju Yeon Park; Oh Yoen Kim; Bo Mi Ham; Hyun-Jin Kim; Dae Young Kwon; Yangsoo Jang; Jong Ho Lee |
Related Documents
:
|
2101828 - Serum ascorbic acid and hdl cholesterol in a healthy elderly japanese population. 13129298 - Consequences of microwave heating and frying on the lipid fraction of chicken and beef ... 638098 - Cord blood hypertriglyceridaemia as an index of fetal stress: use of a simple screening... 956148 - Properties of liposomal membranes composed of short-chain lecithins. 3016558 - Inhibition of human colonic (na+ + k+)-atpase by arachidonic and linoleic acid. 16668228 - Characterization of fatty acid biosynthesis in isolated pea root plastids. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of proteome research Volume: 9 ISSN: 1535-3907 ISO Abbreviation: J. Proteome Res. Publication Date: 2010 Sep |
Date Detail:
|
Created Date: 2010-09-03 Completed Date: 2011-01-04 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101128775 Medline TA: J Proteome Res Country: United States |
Other Details:
|
Languages: eng Pagination: 4368-75 Citation Subset: IM |
Affiliation:
|
Yonsei University Research Institute of Science for Aging, Laboratory of Clinical Nutrigenetics/Nutrigenomics, Department of Food and Nutrition, Brain Korea 21 Project, College of Human Ecology, Yonsei University, Seoul, Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Biological Markers / blood, chemistry Chromatography, High Pressure Liquid / methods* Discriminant Analysis Energy Intake Fatty Acids / blood, chemistry Humans Male Metabolome Metabolomics / methods* Middle Aged Overweight / blood* Phospholipids / blood, chemistry Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods* Statistics, Nonparametric Thinness / blood* |
| Chemical | |
Reg. No./Substance:
|
0/Biological Markers; 0/Fatty Acids; 0/Phospholipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Nickel-catalyzed C-h alkenylation and alkylation of 1,3,4-oxadiazoles with alkynes and styrenes.
Next Document: CO electroxidation on gold in alkaline media: a combined electrochemical, spectroscopic, and DFT stu...