Document Detail


Metabolic profiling of plasma in overweight/obese and lean men using ultra performance liquid chromatography and Q-TOF mass spectrometry (UPLC-Q-TOF MS).
MedLine Citation:
PMID:  20560578     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Obesity is currently epidemic in many countries worldwide and is strongly related to diabetes and cardiovascular disease. This study investigated the differences in metabolomic profiling between overweight/obese and normal-weight men. Overweight/obese (n=30) and age-matched, normal-weight men (n=30) were included. Anthropometric parameters, conventional metabolites, and biomarkers were measured. Metabolomic profiling was analyzed with UPLC-Q-TOF MS. Overweight/obese men showed higher levels of HOMA-IR, triglycerides, total cholesterol, and LDL-cholesterol, and lower levels of HDL-cholesterol and adiponectin than lean men. Overweight/obese men showed higher proportion of stearic acid and lower proportion of oleic acid in serum phospholipids. Additionally, overweight/obese individuals showed higher fat intake and lower ratio of polyunsaturated fatty acids to saturated fatty acids. We identified three lyso-phosphatidylcholine (lysoPC) as potential plasma markers and confirmed eight known metabolites for overweight/obesity men. Especially, overweight/obese subjects showed higher levels of lysoPC C14:0 and lysoPC C18:0 and lower levels of lysoPC C18:1 than lean subjects. Results confirmed abnormal metabolism of two branched-chain amino acids, two aromatic amino acids, and fatty acid synthesis and oxidation in overweight/obese men. Additionally, the amount of dietary saturated fat may influence the proportion of saturated fatty acids in serum phospholipids and the degree of saturation of the constituent acyl group of plasma lysoPC.
Authors:
Ji Young Kim; Ju Yeon Park; Oh Yoen Kim; Bo Mi Ham; Hyun-Jin Kim; Dae Young Kwon; Yangsoo Jang; Jong Ho Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of proteome research     Volume:  9     ISSN:  1535-3907     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-03     Completed Date:  2011-01-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4368-75     Citation Subset:  IM    
Affiliation:
Yonsei University Research Institute of Science for Aging, Laboratory of Clinical Nutrigenetics/Nutrigenomics, Department of Food and Nutrition, Brain Korea 21 Project, College of Human Ecology, Yonsei University, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Adult
Biological Markers / blood,  chemistry
Chromatography, High Pressure Liquid / methods*
Discriminant Analysis
Energy Intake
Fatty Acids / blood,  chemistry
Humans
Male
Metabolome
Metabolomics / methods*
Middle Aged
Overweight / blood*
Phospholipids / blood,  chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Statistics, Nonparametric
Thinness / blood*
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Fatty Acids; 0/Phospholipids

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