Document Detail


Metabolic interactions between the Lands cycle and the Kennedy pathway of glycerolipid synthesis in Arabidopsis developing seeds.
MedLine Citation:
PMID:  23150634     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It has been widely accepted that the primary function of the Lands cycle is to provide a route for acyl remodeling to modify fatty acid (FA) composition of phospholipids derived from the Kennedy pathway. Lysophosphatidylcholine acyltransferase (LPCAT) is an evolutionarily conserved key enzyme in the Lands cycle. In this study, we provide direct evidence that the Arabidopsis thaliana LPCATs, LPCAT1 and LPCAT2, participate in the Lands cycle in developing seeds. In spite of a substantially reduced initial rate of nascent FA incorporation into phosphatidylcholine (PC), the PC level in the double mutant lpcat1 lpcat2-2 remained unchanged. LPCAT deficiency triggered a compensatory response of de novo PC synthesis and a concomitant acceleration of PC turnover that were attributable at least in part to PC deacylation. Acyl-CoA profile analysis revealed complicated metabolic alterations rather than merely reduced acyl group shuffling from PC in the mutant. Shifts in FA stereo-specific distribution in triacylglycerol of the mutant seed suggested a preferential retention of saturated acyl chains at the stereospecific numbering (sn)-1 position from PC and likely a channeling of lysophosphatidic acid, derived from PC, into the Kennedy pathway. Our study thus illustrates an intricate relationship between the Lands cycle and the Kennedy pathway.
Authors:
Liping Wang; Wenyun Shen; Michael Kazachkov; Guanqun Chen; Qilin Chen; Anders S Carlsson; Sten Stymne; Randall J Weselake; Jitao Zou
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-13
Journal Detail:
Title:  The Plant cell     Volume:  24     ISSN:  1532-298X     ISO Abbreviation:  Plant Cell     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2013-01-01     Completed Date:  2013-08-21     Revised Date:  2013-11-05    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4652-69     Citation Subset:  IM    
Affiliation:
National Research Council Canada, Saskatoon, Saskatchewan S7N 0W9, Canada.
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MeSH Terms
Descriptor/Qualifier:
1-Acylglycerophosphocholine O-Acyltransferase / genetics,  metabolism*
Acyl Coenzyme A / metabolism
Acylation
Arabidopsis / enzymology*,  genetics,  growth & development
Fatty Acids / metabolism
Flowers / enzymology,  genetics,  growth & development
Gene Expression Regulation, Plant*
Glycolipids / metabolism
Metabolic Networks and Pathways
Models, Biological
Mutation
Oligonucleotide Array Sequence Analysis
Phenotype
Phosphatidylcholines / metabolism*
Plant Leaves / enzymology,  genetics,  growth & development
Seedling / enzymology,  genetics,  growth & development
Seeds / enzymology*,  genetics,  growth & development
Substrate Specificity
Triglycerides / metabolism
Chemical
Reg. No./Substance:
0/Acyl Coenzyme A; 0/Fatty Acids; 0/Glycolipids; 0/Phosphatidylcholines; 0/Triglycerides; EC 2.3.1.23/1-Acylglycerophosphocholine O-Acyltransferase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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