| Messenger RNA for neuropeptide Y in the arcuate nucleus increases in parallel with plasma adrenocorticotropin during sepsis in the rat. | |
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MedLine Citation:
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PMID: 19383429 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Loss of appetite occurs in the cecal ligation and puncture (CLP) model of sepsis in conjunction with the activation of central neural stress pathways. Neuropeptide Y (NPY) in the arcuate nucleus of the hypothalamus is upregulated by several stressors and is stimulatory to feeding. To examine the response of NPY messenger RNA in the arcuate nucleus to sepsis, we used biotinylated RNA probes and a quantitative non-isotopic in situ hybridization approach in cryo-preserved sections from rats made septic by CLP. The mRNA in arcuate neurons was upregulated from the first day after CLP. By the afternoon of the third day through the morning of the fourth day, the average grey level of NPY mRNA clusters was 30% greater after CLP than after sham surgery (P<0.05), and the integrated optical density based on both the grey level and the amount of area with detectable mRNA was 60% greater after CLP than after sham surgery (P<0.03). Both the average grey level and area with detectable staining were positively correlated to plasma ACTH (r=0.953 and 0.917, respectively, n=10 and P<0.01 in each case). Thus sepsis increases the expression of the mRNA for NPY in the arcuate nucleus in proportion to the magnitude of the stress response. However, the suppression of feeding behavior in the CLP model suggests that sepsis activates additional mechanisms that negate the orexigenic contribution of the neuronal increase in NPY mRNA. |
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Authors:
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Drew E Carlson; Weiwei Le; William C Chiu; Gloria E Hoffman |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2009-01-21 |
Journal Detail:
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Title: Neuroscience letters Volume: 452 ISSN: 0304-3940 ISO Abbreviation: Neurosci. Lett. Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-04-22 Completed Date: 2009-06-29 Revised Date: 2010-09-24 |
Medline Journal Info:
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Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: Ireland |
Other Details:
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Languages: eng Pagination: 146-50 Citation Subset: IM |
Affiliation:
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Department of Surgery, University of Maryland School of Medicine, Baltimore, MD 21201, USA. carlsonde@mail.nih.gov |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adrenal Cortex
/
metabolism,
secretion Adrenal Cortex Hormones / metabolism, secretion Adrenocorticotropic Hormone / blood*, secretion Animals Appetite / physiology Arcuate Nucleus / cytology, metabolism*, secretion Disease Models, Animal Feeding Behavior / physiology Male Neurons / metabolism, secretion Neuropeptide Y / genetics* RNA, Messenger / metabolism Rats Rats, Sprague-Dawley Sepsis / blood*, genetics, physiopathology Stress, Physiological / physiology* Up-Regulation / physiology |
| Grant Support | |
ID/Acronym/Agency:
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GM-063050/GM/NIGMS NIH HHS; NS-28730/NS/NINDS NIH HHS; R01 GM063050-03/GM/NIGMS NIH HHS; R01 GM063050-04/GM/NIGMS NIH HHS; R01 NS028730-13/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Adrenal Cortex Hormones; 0/Neuropeptide Y; 0/RNA, Messenger; 9002-60-2/Adrenocorticotropic Hormone |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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