Document Detail

Messenger RNA electroporation: an efficient tool in immunotherapy and stem cell research.
MedLine Citation:
PMID:  16382887     Owner:  NLM     Status:  MEDLINE    
Over the last decades medicine has developed tremendously, but still many diseases are incurable. The last years, cellular (gene) therapy has become a hot topic in biomedical research for the potential treatment of cancer, AIDS and diseases involving cell loss or degeneration. Here, we will focus on two major areas within cellular therapy, cellular immunotherapy and stem cell therapy, that could benefit from the introduction of neo-expressed genes through mRNA electroporation for basic research as well as for clinical applications. For cellular immunotherapy, we will provide a state-of-the-art on loading antigen-presenting cells with antigens in the mRNA format for manipulation of T cell immunity. In the area of stem cell research, we will highlight current gene transfer methods into adult and embryonic stem cells and discuss the use of mRNA electroporation for controlling guided differentiation of stem cells into specialized cell lineages.
A Van Driessche; P Ponsaerts; D R Van Bockstaele; V F I Van Tendeloo; Z N Berneman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society     Volume:  43     ISSN:  0239-8508     ISO Abbreviation:  Folia Histochem. Cytobiol.     Publication Date:  2005  
Date Detail:
Created Date:  2005-12-30     Completed Date:  2006-03-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8502651     Medline TA:  Folia Histochem Cytobiol     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  213-6     Citation Subset:  IM    
Laboratory of Experimental Hematology, Faculty of Medicine, University of Antwerp, Belgium.
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MeSH Terms
Cell Differentiation / drug effects
Dendritic Cells / metabolism
Electroporation / methods*
Lymphocytes / immunology
RNA, Messenger / administration & dosage*,  genetics
Stem Cells / metabolism*
Reg. No./Substance:
0/RNA, Messenger

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