Document Detail

Mesodermal retinoic acid signaling regulates endothelial cell coalescence in caudal pharyngeal arch artery vasculogenesis.
MedLine Citation:
PMID:  22040871     Owner:  NLM     Status:  MEDLINE    
Disruption of retinoic acid signaling causes a variety of pharyngeal arch artery and great vessel defects, as well as malformations in many other tissues, including those derived from the pharyngeal endoderm. Previous studies implied that arch artery defects in the context of defective RA signaling occur secondary to pharyngeal pouch segmentation defects, although this model has never been experimentally verified. In this study, we examined arch artery morphogenesis during mouse development, and the role of RA in this process. We show in normal embryos that the arch arteries form by vasculogenic differentiation of pharyngeal mesoderm. Using various genetic backgrounds and tissue-specific mutation approaches, we segregate pharyngeal arch artery and pharyngeal pouch defects in RA receptor mutants, and show that RA signal transduction only in pharyngeal mesoderm is required for arch artery formation. RA does not control pharyngeal mesodermal differentiation to endothelium, but instead promotes the aggregation of endothelial cells into nascent vessels. Expression of VE-cadherin was substantially reduced in RAR mutants, and this deficiency may underlie the arch artery defects. The consequences of disrupted mesodermal and endodermal RA signaling were restricted to the 4th and 6th arch arteries and to the 4th pharyngeal pouch, respectively, suggesting that different regulatory mechanisms control the formation of the more anterior arch arteries and pouches.
Peng Li; Mohammad Pashmforoush; Henry M Sucov
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-10-20
Journal Detail:
Title:  Developmental biology     Volume:  361     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2011-12-05     Completed Date:  2012-02-01     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  116-24     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antigens, CD / metabolism
Arteries / embryology*
Branchial Region / blood supply*,  embryology
Cadherins / metabolism
Cell Aggregation / physiology
Cell Differentiation / physiology
Endothelial Cells / cytology,  physiology*
Fluorescent Antibody Technique
Gene Knockout Techniques
In Situ Hybridization
Mesoderm / metabolism*,  physiology
Morphogenesis / physiology*
Mutation / genetics
Receptors, Retinoic Acid / genetics
Signal Transduction / physiology*
Tretinoin / metabolism*
Grant Support
Reg. No./Substance:
0/Antigens, CD; 0/Cadherins; 0/Receptors, Retinoic Acid; 0/cadherin 5; 094ZI81Y45/Tamoxifen; 5688UTC01R/Tretinoin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Effect of interaction between PPARG, PPARA and ADIPOQ gene variants and dietary fatty acids on plasm...
Next Document:  Influence of mesodermal Fgf8 on the differentiation of neural crest-derived postganglionic neurons.