| Mesoderm cell development from ES cells. | |
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MedLine Citation:
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PMID: 20336518 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pluripotent stem cells such as embryonic stem (ES) and induced pluripotent stem (iPS) cells have attractive attention as a source of cells for use in therapeutic application. However, as the in vitro differentiation culture does not provide usefully positional information for cell type definition, this system definitely requires visible markers to identify and monitor the intermediates that present on the way of differentiation. We have been developing the cell surface markers against the various types of mesoderm in the ES cell culture. Using it, we have identified the intermediates of mesoderm and dissected their differentiation pathways in ES cell differentiation. The method described here could be useful for inducing and purifying mesoderm cells from iPS as well as ES cell cultures. |
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Authors:
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Takumi Era |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 636 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-03-25 Completed Date: 2010-06-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 87-103 Citation Subset: IM |
Affiliation:
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Division of Molecular Neurobiology, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan. tera@kumamoto-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers / metabolism Cell Culture Techniques / methods* Cell Differentiation / physiology* Cell Lineage Cells, Cultured Embryo, Mammalian / cytology, physiology Embryonic Stem Cells / cytology, physiology* Mesoderm / cytology* Pluripotent Stem Cells / cytology, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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