Document Detail

Mesoderm cell development from ES cells.
MedLine Citation:
PMID:  20336518     Owner:  NLM     Status:  MEDLINE    
Pluripotent stem cells such as embryonic stem (ES) and induced pluripotent stem (iPS) cells have attractive attention as a source of cells for use in therapeutic application. However, as the in vitro differentiation culture does not provide usefully positional information for cell type definition, this system definitely requires visible markers to identify and monitor the intermediates that present on the way of differentiation. We have been developing the cell surface markers against the various types of mesoderm in the ES cell culture. Using it, we have identified the intermediates of mesoderm and dissected their differentiation pathways in ES cell differentiation. The method described here could be useful for inducing and purifying mesoderm cells from iPS as well as ES cell cultures.
Takumi Era
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  636     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-03-25     Completed Date:  2010-06-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  87-103     Citation Subset:  IM    
Division of Molecular Neurobiology, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan.
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MeSH Terms
Biological Markers / metabolism
Cell Culture Techniques / methods*
Cell Differentiation / physiology*
Cell Lineage
Cells, Cultured
Embryo, Mammalian / cytology,  physiology
Embryonic Stem Cells / cytology,  physiology*
Mesoderm / cytology*
Pluripotent Stem Cells / cytology,  physiology*
Reg. No./Substance:
0/Biological Markers

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