Document Detail


Mesoangioblasts from ventricular vessels can differentiate in vitro into cardiac myocytes with sinoatrial-like properties.
MedLine Citation:
PMID:  19837079     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cardiac mesoangioblasts (MABs) are a class of vessel-associated clonogenic, self-renewing progenitor cells, recently identified in the post-natal murine heart and committed to cardiac differentiation. Cardiomyocytes generated during cardiogenesis from progenitor cells acquire several distinct phenotypes, corresponding to different functional properties in diverse structures of the adult heart. Given the special functional relevance to rhythm generation and rate control of sinoatrial cells, and in view of their prospective use in therapeutical applications, we sought to determine if, and to what extent, cardiac mesoangioblasts could also differentiate into myocytes with properties typical of mature pacemaker myocytes. We report here that a subpopulation of cardiac mesoangioblasts, induced to differentiate in vitro into cardiomyocytes, do acquire a phenotype with specific mature pacemaker myocytes properties. These include expression of the HCN4 isoform of pacemaker ("funny", f-) channels and connexin 45 (Cx45), as well as reduced expression of inwardly-rectifying potassium channels. Furthermore, MAB-derived myocytes form agglomerates of pacing cells displaying stable rhythmic activity, and as in native cardiac pacemaker cells, f-channel modulation by autonomic transmitters contributes to control of spontaneous rate in differentiated mesoangioblasts. These data represent the first evidence for in vitro generation of pacemaker-like myocytes from proliferating non-embryonic stem/progenitor cells.
Authors:
Andrea Barbuti; Beatriz G Galvez; Alessia Crespi; Angela Scavone; Mirko Baruscotti; Chiara Brioschi; Giulio Cossu; Dario DiFrancesco
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-22
Journal Detail:
Title:  Journal of molecular and cellular cardiology     Volume:  48     ISSN:  1095-8584     ISO Abbreviation:  J. Mol. Cell. Cardiol.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-28     Completed Date:  2010-04-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0262322     Medline TA:  J Mol Cell Cardiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  415-23     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Biomolecular Sciences and Biotechnology, The PaceLab, University of Milano, via Celoria 26, 20133 Milan, Italy. andrea.barbuti@unimi.it
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / metabolism
Blood Vessels / cytology*
Cell Differentiation*
Clone Cells
Cyclic Nucleotide-Gated Cation Channels / metabolism
GATA6 Transcription Factor / metabolism
Heart Ventricles / cytology*
Humans
Ion Channel Gating
Mice
Mice, Inbred C57BL
Myocytes, Cardiac / cytology*,  metabolism
Protein Isoforms / metabolism
Receptors, Adrenergic, beta / metabolism
Receptors, Cholinergic / metabolism
Sinoatrial Node / cytology*
Stem Cells / cytology*,  metabolism
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Cyclic Nucleotide-Gated Cation Channels; 0/GATA6 Transcription Factor; 0/Protein Isoforms; 0/Receptors, Adrenergic, beta; 0/Receptors, Cholinergic

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