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Mesenteric lymph node B lymphoblasts which home to the small intestine are precommitted to IgA synthesis.
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MedLine Citation:
PMID:  300777     Owner:  NLM     Status:  MEDLINE    
The fate of mesenteric lymph node lymphoblasts labeled with either [125I]iododeoxyuridine or [3H]thymidine can be studied after intravenous transfer into syngeneic mice both by measurement of radioactivity in various organs and by combined immunofluorescence and autoradiography of recipient tissues. Many of the lymphoblasts home to the lamina propria of the small intestine within hours of transfer; of these, many visibly secrete IgA. To determine whether the cells that will ultimately secrete IgA are already committed to IgA synthesis before their arrival in the gut, mesenteric lymph node cell populations were treated with various class-specific antisera to mouse immunoglobulins before transfer. Treatment with antiserum to IgA, plus complement, reduced the fraction of injected label recovered from the recipients' intestines, and also reduced the proportion of donor (labeled) cells containing IgA. We conclude that mesenteric lymph nodes are probably the principal source of IgA-secreting plasma cells in the lamina propria of the gut, and that the cells become committed to IgA synthesis and develop cell surface IgA before emigrating. This IgA is apparently synthesized by the cells that bear it since it is not removed by extensive rinsing at 37 degrees C, a maneuver that elutes passively adsorbed immunoglobulin.
M McWilliams; J M Phillips-Quagliata; M E Lamm
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of experimental medicine     Volume:  145     ISSN:  0022-1007     ISO Abbreviation:  J. Exp. Med.     Publication Date:  1977 Apr 
Date Detail:
Created Date:  1977-06-30     Completed Date:  1977-06-30     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985109R     Medline TA:  J Exp Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  866-75     Citation Subset:  AIM; IM    
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MeSH Terms
Antibodies, Anti-Idiotypic
B-Lymphocytes / immunology*
Cell Differentiation
Immunoglobulin A / biosynthesis*
Intestine, Small / immunology*
Lymph Nodes / immunology
Mice, Inbred BALB C
Plasma Cells / cytology,  immunology
Receptors, Antigen, B-Cell / analysis
Reg. No./Substance:
0/Antibodies, Anti-Idiotypic; 0/Immunoglobulin A; 0/Receptors, Antigen, B-Cell

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): J Exp Med
ISSN: 0022-1007
ISSN: 1540-9538
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 4 Year: 1977
Volume: 145 Issue: 4
First Page: 866 Last Page: 875
ID: 2180636
Publisher Id: 77166993
PubMed Id: 300777

Mesenteric lymph node B lymphoblasts which home to the small intestine are precommitted to IgA synthesis

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