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Mesenchymal stem cells restore CCl4-induced liver injury by an antioxidative process.
MedLine Citation:
PMID:  23035905     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We studied bone marrow (BM)-derived mesenchymal stem cells (MSCs) for the treatment of liver injury. We hypothesized that MSC-mediated resolution of liver injury could occurred through an antioxidative process. After being injected with carbon tetrachloride (CCl4), mice were injected with syngenic BM-derived MSCs or normal saline. Oxidative stress activity of the MSCs was determined by analysis of reactive oxygen species (ROS) and superoxide dismutase (SOD) activity. In addition, cytoprotective genes of liver tissue were assessed by real-time PCR and an antioxidant response element reporter (ARE) assay. Upregulated ROS of CCl4-treated liver cells was attenuated by co-culturing with MSCs. Blockage of SOD by adding an SOD inhibitor decreased the effect of MSCs on injured liver cells. MSCs caused a significant increase in SOD activity and inhibited ROS production in the injured liver. The gene expression levels of heme oxygenase-1, BI-1, hepatocyte growth factor, glutathione S-transferase, and nuclear factor erythroid 2-related factor 2, attenuated by CCl4, were increased up to basal levels after MSC transplantation, as shown by real-time polymerase chain reaction. In addition, MSCs induced an antioxidant response element, shown with luciferase activity, which represented a cytoprotective response in injured liver. Our results provide evidence of a new cytoprotective effect in which MSCs promote an antioxidant response and support the potential of using MSC transplantation as an effective treatment modality for liver disease.
Authors:
Kyung-Ah Cho; So-Youn Woo; Ju-Young Seoh; Ho-Seong Han; Kyung-Ha Ryu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-5
Journal Detail:
Title:  Cell biology international     Volume:  -     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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