Document Detail


Mesenchymal stem cells overexpressing MiR-126 enhance ischemic angiogenesis via the AKT/ERK-related pathway.
MedLine Citation:
PMID:  22113756     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Background: This study was designed to examine whether transplantation of mesenchymal stem cells (MSCs) overexpressing miR-126 enhances angiogenesis in the infarcted myocardium of mice. Methods: MSCs were harvested from mice using density gradient centrifugation and adherent culture. MSCs were transfected with lentiviral vectors carrying mature miR-126. Mice models of myocardial infarction were established by ligation of coronary artery. The ligated animals were randomly divided into three groups (15 in each) and after two weeks, were intramyocardially injected at the heart infarct zone with miR-126-transfected MSCs (the miR-126-MSCs group), MSCs (the MSCs group), or medium (the PBS group). Six weeks later, histological study and echocardiographic assessment were performed. Results: Capillary density of the infarcted region was significantly improved in the miR-126- MSCs group compared to the MSC group and the PBS group (both p 〈 0.01). Western blot showed that ERK(1), pERK(1), AKT and pAKT gene were dramatically enhanced in the miR-126-MSC group compared to the MSC group and the PBS group (both p 〈 0.05). Echocardiography showed MiR-126 led to a sustained improvement in cardiac function for at least six weeks at the injected area, as assessed by left ventricular ejection fraction and fraction of shortening. Conclusions: Transplantation of MSCs transfected with miR-126 can improve angiogenesis and cardiac function in the infarcted area of the hearts of mice, which may be due to stimulation of the AKT/ERK-related pathway. (Cardiol J 2011; 18, 6: 675-681).
Authors:
Jian-Jun Chen; Sheng-Hua Zhou
Related Documents :
2372796 - Heat production by the human left ventricle: measurement by a thermodilution technique.
2354706 - Transcutaneous nerve stimulation in patients with coronary arterial disease: haemodynam...
6630586 - Myocardial metabolic effects of intravenous terbutaline in patients with severe heart f...
12890186 - Cardiac energetics: sense and nonsense.
8770316 - Acute supplementation with the nitric oxide precursor l-arginine does not improve cardi...
16020596 - Comparison of the effect of enalapril and losartan in conjunction with surgical coronar...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cardiology journal     Volume:  18     ISSN:  1897-5593     ISO Abbreviation:  Cardiol J     Publication Date:  2011  
Date Detail:
Created Date:  2011-11-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101392712     Medline TA:  Cardiol J     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  675-81     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Half of coronary patients are not instructed how to respond to symptoms of a heart attack.
Next Document:  Acute alcohol consumption is associated with increased interatrial electromechanical delay in health...