Document Detail


Mesenchymal stem cells isolated from human uterine cervix cancer tissues.
MedLine Citation:
PMID:  20874707     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the present study, MSCs (mesenchymal stem cells) were successfully isolated and identified from hUCC (human uterine cervix cancer) tissues. The morphological appearance, immunophenotype, growth curve, cell cycle, cytogenetic features and differentiation potential of these cells were investigated. Results showed that cells isolated from the uterine cervix cancer tissues displayed fibroblast-like morphology and grew into colonies. Immunophenotyping by flow cytometry revealed that the isolated cells were positive for CD13, CD29, CD44, CD105 and HLA-I, while negative for CD10, CD14, CD31, CD34, CD38 and HLA-DR. The cells kept a normal karyotype by chromosome analysis. At the third passage, the percentages of cells in G0-/G1-, 2-/M- and S-phase were 84.94, 8.36 and 6.71%, respectively. Under appropriate induction conditions, these cells can differentiate into osteogenic, adipogenic cells and hepatocytes. Taken together, MSCs were confirmed to exist in hUCC tissues, which may provide a new target for clinical cancer therapy.
Authors:
Xiaochun Sun; Hua Cai; Hui Qian; Wei Zhu; Yongmin Yan; Huaxi Xu; Wenrong Xu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international     Volume:  35     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-25     Completed Date:  2011-05-03     Revised Date:  2012-08-02    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  119-23     Citation Subset:  IM    
Affiliation:
School of Medical Science and Laboratory Medicine, Key Institute of Clinical Laboratory Science, Jiangsu University, Zhenjiang Key Institute of Clinical Laboratory Medicine, 301 Xuefu Road, Zhenjiang City, Jiangsu Province 212013, Peoples Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD / analysis
Cell Differentiation
Cell Division
Cells, Cultured
Cervix Uteri / pathology*
Female
Humans
Immunophenotyping
Interphase
Karyotyping
Mesenchymal Stem Cells / pathology*
Uterine Cervical Neoplasms / pathology*
Chemical
Reg. No./Substance:
0/Antigens, CD

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