| Mesenchymal stem cells from umbilical cord blood: parameters for isolation, characterization and adipogenic differentiation. | |
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MedLine Citation:
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PMID: 22328147 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Isolation of mesenchymal stem cells (MSCs) from umbilical cord blood (UCB) from full-term deliveries is a laborious, time-consuming process that results in a low yield of cells. In this study we identified parameters that can be helpful for a successful isolation of UCB-MSCs. According to our findings, chances for a well succeeded isolation of these cells are higher when MSCs were isolated from UCB collected from normal full-term pregnancies that did not last over 37 weeks. Besides the duration of pregnancy, blood volume and storage period of the UCB should also be considered for a successful isolation of these cells. Here, we found that the ideal blood volume collected should be above 80 mL and the period of storage should not exceed 6 h. We characterized UCB-MSCs by morphologic, immunophenotypic, protein/gene expression and by adipogenic differentiation potential. Isolated UCB-MSCs showed fibroblast-like morphology and the capacity of differentiating into adipocyte-like cells. Looking for markers of the undifferentiated status of UCB-MSCs, we analyzed the UCB-MSCs' protein expression profile along different time periods of the differentiation process into adipocyte-like cells. Our results showed that there is a decrease in the expression of the markers CD73, CD90, and CD105 that correlates to the degree of differentiation of UCB-MSCs We suggest that CD90 can be used as a mark to follow the differentiation commitment degree of MSCs. Microarray results showed an up-regulation of genes related to the adipogenesis process and to redox metabolism in the adipocyte-like differentiated MSCs. Our study provides information on a group of parameters that may help with successful isolation and consequently with characterization of the differentiated/undifferentiated status of UCB-MSCs, which will be useful to monitor the differentiation commitment of UCB-MSC and further facilitate the application of those cells in stem-cell therapy. |
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Authors:
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Tatiana Taís Sibov; P Severino; L C Marti; L F Pavon; D M Oliveira; P R Tobo; A H Campos; A T Paes; E Amaro; L F Gamarra; C A Moreira-Filho |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-2-12 |
Journal Detail:
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Title: Cytotechnology Volume: - ISSN: 0920-9069 ISO Abbreviation: - Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-2-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8807027 Medline TA: Cytotechnology Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Instituto do Cérebro, Instituto Israelita de Ensino e Pesquisa Albert Einstein-IIEPAE, Hospital Israelita Albert Einstein, Avenida Albert Einstein, no 627/701, Morumbi, São Paulo, SP, CEP: 05651-901, Brazil, tatianats@einstein.br. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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