Document Detail


Mesenchymal stem cells from murine amniotic fluid as a model for preclinical investigation.
MedLine Citation:
PMID:  21361715     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Despite the suitability of a mouse model for preclinical investigations; little is known regarding mesenchymal stem cells derived from murine amniotic fluid. This is the subject of the present study. 
METHODS:   Amniotic fluid was collected from NMRI mice during the second weeks of pregnancy and plated. The cells that adhered to the culture surfaces were propagated with three successive subcultures and then characterized. To determine the differentiation potential, the cells were cultivated under osteogenic, adipogenic, and chondrogenic conditions, and followed by specific staining and RT-PCR analysis for differentiation. The proliferative potential of the cells were measured with clonogenic assays, population doubling time and number and by growth curve plotting. Cellular aging was investigated with the senescence-associated ß-galactosidase staining method.
RESULTS: The amniotic fluid primary cell culture was composed of round flattened and fibroblastic cells. The latter dominated the culture after several passages. Successful tripotent differentiation of the isolated cells into bone, cartilage and adipose cells were indicative of their mesenchymal stem cells nature. The isolated cells appeared to be relatively proliferative cells as confirmed by the population doubling time value which was equal to about 69 hours. Furthermore, the cells were relatively clonogenic and they tended to initiate proliferation immediately after plating (there was no lag phase in their growth curve). ß-galactosidase positive cells were first observed at passage 3 and increased in number with subsequent passages.
CONCLUSIONS: Collectively it was concluded that murine amniotic fluid contained mesenchymal stem cells with relatively high proliferation property and typical tripotent differentiation potential.  
Authors:
Mohamadreza Baghaban Eslaminejad; Shahrbanoo Jahangir; Nasser Aghdami
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of Iranian medicine     Volume:  14     ISSN:  1735-3947     ISO Abbreviation:  Arch Iran Med     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-02     Completed Date:  2011-07-22     Revised Date:  2013-05-15    
Medline Journal Info:
Nlm Unique ID:  100889644     Medline TA:  Arch Iran Med     Country:  Iran    
Other Details:
Languages:  eng     Pagination:  96-103     Citation Subset:  IM    
Affiliation:
Department of Stem Cell and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran. eslami@royaninstitute.org
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MeSH Terms
Descriptor/Qualifier:
Amniotic Fluid / cytology*
Animals
Antigens, CD44 / metabolism
Antigens, Thy-1 / metabolism
Cell Aging*
Cell Differentiation*
Cell Proliferation*
Cells, Cultured
Female
Flow Cytometry
Mesenchymal Stromal Cells / cytology*,  metabolism
Mice
Models, Animal
Models, Biological
Reverse Transcriptase Polymerase Chain Reaction
beta-Galactosidase / metabolism
Chemical
Reg. No./Substance:
0/Antigens, CD44; 0/Antigens, Thy-1; EC 3.2.1.23/beta-Galactosidase

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