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Mesenchymal stem cells derived from different origins have unique sensitivities to different chemotherapeutic agents.
MedLine Citation:
PMID:  22694597     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Bone-marrow-derived mesenchymal stem cells(BMSCs)and adipose-tissue-derived mesenchymal stem cells (ADSCs)are virtually identical in cell surface marker profile and differentiation potential. These cell populations have promising characteristics for clinical application. In this study, we evaluated the sensitivity of these cell populations to various chemotherapeutic agents by testing the inhibition of cell proliferation, low molecular DNA bands formation, in situ apoptosis, apoptosis related gene expression and cell senescence after treatment with these agents. Our results demonstrated that busulfan, methotrexate and doxorubicin treatments led to a marked and dose-dependent reduction in cell viability when compared with 5-fluorouracil treatment. Different expression patterns of apoptosis-related genes were found in the BMSCs and ADSCs following treatment with the agents, but no low molecular weight DNA bands were detected. BMSCs demonstrated a higher percentage of apoptotic and senescent cells following treatment with chemotherapeutic agents compared with ADSCs. These findings suggest that these two cell populations respond differently to chemotherapy treatment, ADSCs exhibit more resistant than BMSCs to chemotherapy treatment induced cell senescence and apoptosis, indicating it might be more advantageous to use in the clinic than BMSCs.
Authors:
Zhengyu Qi; Yanmin Zhang; Lin Liu; Xin Guo; Jie Qin; Guanghui Cui
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-14
Journal Detail:
Title:  Cell biology international     Volume:  -     ISSN:  1095-8355     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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