Document Detail


Mesenchymal stem cells contribute to endogenous FVIII:c production.
MedLine Citation:
PMID:  23042590     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Besides the liver, it has been difficult to identify which organ(s) and/or cellular component(s) contribute significantly to the production of human FVIII:c (FVIII). Thus far, only endothelial cells have been shown to constitute a robust extrahepatic source of FVIII, possibly explaining both the diverse presence of FVIII mRNA in the body, and the observed increase in FVIII levels during liver failure. Here, we investigate whether human mesenchymal stem cells (MSC), ubiquitously present in different organs, could also contribute to FVIII production. MSC isolated from human lung, liver, brain, and bone marrow expressed FVIII message as determined by quantitative-RT-PCR. Using an antibody specific for FVIII, confocal microscopy, and umbilical cord-derived endothelial cells (HUVEC) as a negative control, we demonstrated that, in MSC, FVIII protein was not stored in granules; rather, it localized to the perinuclear region. Furthermore, functional FVIII was detected in MSC supernatants and cell lysates by aPTT and chromogenic assays. These results demonstrate that MSC can contribute at low levels to the functional FVIII pool, and advance the understanding of the physiology of FVIII production and secretion.
Authors:
Chad Sanada; Chung-Jung Kuo; Evan J Colletti; Melisa Soland; Saloomeh Mokhtari; Mary Ann Knovich; John Owen; Esmail D Zanjani; Christopher D Porada; Graça Almeida-Porada
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  228     ISSN:  1097-4652     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-01-28     Completed Date:  2013-04-03     Revised Date:  2014-03-27    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1010-6     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cell Differentiation
Endothelial Cells / cytology,  metabolism
Factor VIII / biosynthesis*,  secretion*
Human Umbilical Vein Endothelial Cells
Humans
Mesenchymal Stromal Cells* / metabolism,  secretion
Microscopy, Confocal
RNA, Messenger / metabolism
Secretory Vesicles / metabolism
Tissue Distribution
Grant Support
ID/Acronym/Agency:
HL73737/HL/NHLBI NIH HHS; HL97623/HL/NHLBI NIH HHS; R01 HL073737/HL/NHLBI NIH HHS; R01 HL097623/HL/NHLBI NIH HHS; T32 HL007974/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/F8 protein, human; 0/RNA, Messenger; 9001-27-8/Factor VIII
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A signal transduction score flow algorithm for cyclic cellular pathway analysis, which combines tran...
Next Document:  Fiber type-specific differences in glucose uptake by single fibers from skeletal muscles of 9- and 2...