Document Detail

Mesenchymal stem cells can improve anal pressures after anal sphincter injury.
MedLine Citation:
PMID:  23147650     Owner:  NLM     Status:  Publisher    
OBJECTIVE.: Fecal incontinence reduces the quality of life of many women but has no long-term cure. Research on mesenchymal stem cell (MSC)-based therapies has shown promising results. The primary aim of this study was to evaluate functional recovery after treatment with MSCs in two animal models of anal sphincter injury. METHODS.: Seventy virgin female rats received a sphincterotomy (SP) to model episiotomy, a pudendal nerve crush (PNC) to model the nerve injuries of childbirth, a sham SP, or a sham PNC. Anal sphincter pressures and electromyography (EMG) were recorded after injury but before treatment and 10days after injury. Twenty-four hours after injury, each animal received either 0.2ml saline or 2million MSCs labelled with green fluorescing protein (GFP) suspended in 0.2ml saline, either intravenously (IV) into the tail vein or intramuscularly (IM) into the anal sphincter. RESULTS.: MSCs delivered IV after SP resulted in a significant increase in resting anal sphincter pressure and peak pressure, as well as anal sphincter EMG amplitude and frequency 10days after injury. MSCs delivered IM after SP resulted in a significant increase in resting anal sphincter pressure and anal sphincter EMG frequency but not amplitude. There was no improvement in anal sphincter pressure or EMG with in animals receiving MSCs after PNC. GFP-labelled cells were not found near the external anal sphincter in MSC-treated animals after SP. CONCLUSION.: MSC treatment resulted in significant improvement in anal pressures after SP but not after PNC, suggesting that MSCs could be utilized to facilitate recovery after anal sphincter injury.
Levilester Salcedo; Maritza Mayorga; Margot Damaser; Brian Balog; Robert Butler; Marc Penn; Massarat Zutshi
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-16
Journal Detail:
Title:  Stem cell research     Volume:  10     ISSN:  1876-7753     ISO Abbreviation:  Stem Cell Res     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-11-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101316957     Medline TA:  Stem Cell Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  95-102     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Department of Colorectal Surgery, Cleveland Clinic, Cleveland, OH, USA.
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