Document Detail


Mesenchymal cell fusion in the sea urchin embryo.
MedLine Citation:
PMID:  18979252     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mesenchymal cells of the sea urchin embryo provide a valuable experimental model for the analysis of cell-cell fusion in vivo. The unsurpassed optical transparency of the sea urchin embryo facilitates analysis of cell fusion in vivo using fluorescent markers and time-lapse three-dimensional imaging. Two populations of mesodermal cells engage in homotypic cell-cell fusion during gastrulation: primary mesenchyme cells and blastocoelar cells. In this chapter, we describe methods for studying the dynamics of cell fusion in living embryos. These methods have been used to analyze the fusion of primary mesenchyme cells and are also applicable to blastocoelar cell fusion. Although the molecular basis of cell fusion in the sea urchin has not been investigated, tools have recently become available that highlight the potential of this experimental model for integrating dynamic morphogenetic behaviors with underlying molecular mechanisms.
Authors:
Paul G Hodor; Charles A Ettensohn
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  475     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-11-03     Completed Date:  2008-11-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  315-34     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blastomeres / cytology,  drug effects
Cell Fusion / methods*
Embryo, Nonmammalian / cytology*,  drug effects,  ultrastructure
Fluorescent Dyes / pharmacology
Mesoderm / cytology*,  drug effects,  transplantation
Microinjections
Sea Urchins / cytology*,  drug effects,  embryology*,  ultrastructure
Stem Cells / cytology,  drug effects
Chemical
Reg. No./Substance:
0/Fluorescent Dyes

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