| Mesenchymal stem cell and nucleus pulposus cell coculture modulates cell profile. | |
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MedLine Citation:
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PMID: 19034596 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Spontaneous cell fusion can occur in cocultured stem cells. We examined whether telomerase activity change and cell fusion occurred in mesenchymal stem cell (MSC) and nucleus pulposus cell (NPC) coculture. MSCs and NPCs were labeled with PKH26 and PKH67 dyes and cocultured at a 50:50 ratio. An equal number of MSCs or NPCs were used as the control. After 14 days, cells were evaluated by cell growth, telomerase activity, quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), immunohistochemistry, and histologic observation. Cell fusion was confirmed by microscopic observation and fluorescence-activated cell sorter (FACS) analysis. The results suggested cell growth rate and telomerase activity were higher in cocultured cells than in NPCs cultured alone. The mRNA expression levels of the Type II collagen and aggrecan were elevated in cocultured cells. Immunohistochemical analysis revealed positive staining for Type II collagen and keratan sulfate in NPCs cultured alone and in a proportion of cocultured cells. Histologic observation revealed binucleated cocultured cells expressed both PKH dyes in the same location and slide focus. The FACS analysis revealed 42% of cocultured cells were double-stained. Cocultured cells partially maintained the NPC phenotype. The partially maintained phenotype of the NPCs may be attributable to spontaneous cell fusion in association with increased telomerase activity. |
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Authors:
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Chi-Chien Niu; Li-Jen Yuan; Song-Shu Lin; Lih-Huei Chen; Wen-Jer Chen |
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Publication Detail:
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Type: Journal Article Date: 2008-11-26 |
Journal Detail:
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Title: Clinical orthopaedics and related research Volume: 467 ISSN: 1528-1132 ISO Abbreviation: Clin. Orthop. Relat. Res. Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-11-05 Completed Date: 2010-01-05 Revised Date: 2011-03-03 |
Medline Journal Info:
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Nlm Unique ID: 0075674 Medline TA: Clin Orthop Relat Res Country: United States |
Other Details:
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Languages: eng Pagination: 3263-72 Citation Subset: AIM; IM |
Affiliation:
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Department of Orthopaedic Surgery, Chang Gung Memorial Hospital, No. 5, Fu-Hsing Street 333, Kweishan, Taoyuan, Taiwan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aggrecans
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genetics Animals Biological Markers / metabolism Bone Marrow Cells / enzymology, physiology* Cell Communication* Cell Fusion Cell Proliferation Cell Separation Cells, Cultured Coculture Techniques Collagen Type II / genetics, metabolism Flow Cytometry Immunohistochemistry Intervertebral Disk / cytology, enzymology, physiology* Keratan Sulfate / metabolism Mesenchymal Stem Cells / enzymology, physiology* Microscopy, Fluorescence Phenotype RNA, Messenger / metabolism Rabbits Reverse Transcriptase Polymerase Chain Reaction Telomerase / metabolism Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Aggrecans; 0/Biological Markers; 0/Collagen Type II; 0/RNA, Messenger; 9056-36-4/Keratan Sulfate; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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