Document Detail


Mesenchymal stem cell and nucleus pulposus cell coculture modulates cell profile.
MedLine Citation:
PMID:  19034596     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Spontaneous cell fusion can occur in cocultured stem cells. We examined whether telomerase activity change and cell fusion occurred in mesenchymal stem cell (MSC) and nucleus pulposus cell (NPC) coculture. MSCs and NPCs were labeled with PKH26 and PKH67 dyes and cocultured at a 50:50 ratio. An equal number of MSCs or NPCs were used as the control. After 14 days, cells were evaluated by cell growth, telomerase activity, quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), immunohistochemistry, and histologic observation. Cell fusion was confirmed by microscopic observation and fluorescence-activated cell sorter (FACS) analysis. The results suggested cell growth rate and telomerase activity were higher in cocultured cells than in NPCs cultured alone. The mRNA expression levels of the Type II collagen and aggrecan were elevated in cocultured cells. Immunohistochemical analysis revealed positive staining for Type II collagen and keratan sulfate in NPCs cultured alone and in a proportion of cocultured cells. Histologic observation revealed binucleated cocultured cells expressed both PKH dyes in the same location and slide focus. The FACS analysis revealed 42% of cocultured cells were double-stained. Cocultured cells partially maintained the NPC phenotype. The partially maintained phenotype of the NPCs may be attributable to spontaneous cell fusion in association with increased telomerase activity.
Authors:
Chi-Chien Niu; Li-Jen Yuan; Song-Shu Lin; Lih-Huei Chen; Wen-Jer Chen
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Publication Detail:
Type:  Journal Article     Date:  2008-11-26
Journal Detail:
Title:  Clinical orthopaedics and related research     Volume:  467     ISSN:  1528-1132     ISO Abbreviation:  Clin. Orthop. Relat. Res.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-05     Completed Date:  2010-01-05     Revised Date:  2013-06-04    
Medline Journal Info:
Nlm Unique ID:  0075674     Medline TA:  Clin Orthop Relat Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3263-72     Citation Subset:  AIM; IM    
Affiliation:
Department of Orthopaedic Surgery, Chang Gung Memorial Hospital, No. 5, Fu-Hsing Street 333, Kweishan, Taoyuan, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Aggrecans / genetics
Animals
Biological Markers / metabolism
Bone Marrow Cells / enzymology,  physiology*
Cell Communication*
Cell Fusion
Cell Proliferation
Cell Separation
Cells, Cultured
Coculture Techniques
Collagen Type II / genetics,  metabolism
Flow Cytometry
Immunohistochemistry
Intervertebral Disc / cytology,  enzymology,  physiology*
Keratan Sulfate / metabolism
Mesenchymal Stromal Cells / enzymology,  physiology*
Microscopy, Fluorescence
Phenotype
RNA, Messenger / metabolism
Rabbits
Reverse Transcriptase Polymerase Chain Reaction
Telomerase / metabolism
Time Factors
Chemical
Reg. No./Substance:
0/Aggrecans; 0/Biological Markers; 0/Collagen Type II; 0/RNA, Messenger; 9056-36-4/Keratan Sulfate; EC 2.7.7.49/Telomerase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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