Document Detail

Mesangial cells connected by the N-cadherin-catenin system in the rat kidney.
MedLine Citation:
PMID:  19546590     Owner:  NLM     Status:  MEDLINE    
BACKGROUND/AIMS: Mesangial cells bear the tensional forces generated in the glomerular capillary wall. Not only mesangial cells per se, but also their intercellular junctions should be physically stable against the tensional forces; this prompted the search for actin filament-reinforced adherens junctions of mesangial cells. We previously reported alpha- and beta-catenins localized at the cell-cell contact sites of mesangial cells in the rat. Classical cadherin expressed by mesangial cells, however, remains to be elucidated. METHODS: Expression of classical cadherins, especially N-cadherin, was examined in rat glomeruli by ribonuclease protection assay, Western blot analysis and immunofluorescence and immunoelectron microscopy. RESULTS: Ribonuclease protection assay detected significant expression of N-cadherin in rat glomeruli. Western blot analysis showed that rabbit and murine antibodies against N-cadherin reacted with a specific band in isolated glomeruli. Immunofluorescence microscopy revealed that both antibodies reacted only with the mesangium in glomeruli. Immunoelectron microscopy demonstrated that the immunogold particles for N-cadherin were found predominantly at cell-cell contact sites of mesangial cells where actin filaments concentrated. CONCLUSION: N-cadherin interconnects mesangial cells, suggesting that the cadherin-catenin-actin filament system in the mesangium may play a role in the counteraction of the hydraulic pressure gradient across the capillary wall.
Masaaki Nameta; Eishin Yaoita; Nobutaka Kato; Linning Zhao; Ying Zhang; Hidehiko Fujinaka; Bo Xu; Yutaka Yoshida; Tadashi Yamamoto
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-16
Journal Detail:
Title:  Nephron. Experimental nephrology     Volume:  112     ISSN:  1660-2129     ISO Abbreviation:  Nephron Exp. Nephrol.     Publication Date:  2009  
Date Detail:
Created Date:  2009-08-06     Completed Date:  2010-01-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101159770     Medline TA:  Nephron Exp Nephrol     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  e92-8     Citation Subset:  IM    
Copyright Information:
Copyright 2009 S. Karger AG, Basel.
Cooperative Laboratory of Electron Microscopy, Niigata University, Niigata, Japan.
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MeSH Terms
Blotting, Western
Cadherins / metabolism*
Catenins / metabolism*
Kidney Glomerulus / cytology,  metabolism*,  ultrastructure
Mesangial Cells / cytology,  metabolism,  ultrastructure*
Microscopy, Fluorescence
Microscopy, Immunoelectron
Rats, Inbred WKY
Reg. No./Substance:
0/Cadherins; 0/Catenins

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