Document Detail


Meristem transformation of sunflower via Agrobacterium.
MedLine Citation:
PMID:  24226429     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
For transformation of sunflower (Helianthus annuus L. cv. Zebulon), shoot apical meristems were dissected from seeds and cocultivated with a disarmed Agrobacterium tumefaciens strain harboring a binary vector carrying genes encoding GUS- and NPTII-activity. The influence of the media conditions, the time of cocultivation and the stage of the developing seed on shoot development and meristem transformation was analysed. Transformants were selected by their ability to grow on kanamycin. Transformation was confirmed by assays for GUS and NPTII. GUS-positive shoots were rooted on rockwool and transferred to soil. Transformation of shoot meristem cells occurred at low frequencies. Chimaeric expression of the two genes was observed in transformed plants. Integration of the foreign DNA in the sunflower genome was confirmed with the polymerase chain reaction.
Authors:
B Schrammeijer; P C Sijmons; P J van den Elzen; A Hoekema
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant cell reports     Volume:  9     ISSN:  0721-7714     ISO Abbreviation:  Plant Cell Rep.     Publication Date:  1990 Jul 
Date Detail:
Created Date:  2013-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9880970     Medline TA:  Plant Cell Rep     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  55-60     Citation Subset:  -    
Affiliation:
MOGEN International NV, Einsteinweg 97, 2333 CB, Leiden, The Netherlands.
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