| Mercury accumulation in kidney lysosomes or proteinuric rats. | |
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MedLine Citation:
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PMID: 7230609 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purpose of the present study was to determine whether lysosomal accumulation of mercury in the kidney is due to a leakage of protein-bound mercury through the glomerular filtration barrier followed by reabsorption into the lysosomal system of the proximal tubule. The subcellular distribution of mercury in the kidney was studied in four different groups of rats with and without proteinuria: normal young rats, young rats with aminonucleoside nephrosis, old rats with spontaneous proteinuria, and old rats with chronic mercury intoxication and proteinuria. Radioactive mercuric chloride (203HgCl2) was injected s.c. into the rats 72 hours before sacrifice. Cell fractionation experiments were carried out on homogenates of the renal cortex by differential centrifugation. Determination of radioactive mercury in the subcellular fractions revealed that mercury was concentrated in the lysosomal fraction of all rats with proteinuria. In contrast, normal rats without proteinuria had the highest concentration of mercury in the supernatant, and there was no enrichment of mercury in the lysosomal fraction. Gel filtration chromatography performed on urine samples from proteinuric rats demonstrated that excreted mercury in renal lysosomes of proteinuric urine support the hypothesis that mercury bound to plasma proteins passes the glomerular filtration barrier in proteinuric conditions and enters the lysosomal system of the proximal tubule by way of endocytosis. |
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Authors:
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K M Madsen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Kidney international Volume: 18 ISSN: 0085-2538 ISO Abbreviation: Kidney Int. Publication Date: 1980 Oct |
Date Detail:
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Created Date: 1981-07-09 Completed Date: 1981-07-09 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0323470 Medline TA: Kidney Int Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 445-53 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Albumins
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metabolism Albuminuria Animals Chromatography, Gel Kidney / metabolism*, ultrastructure Kidney Glomerulus / metabolism Kidney Tubules, Proximal / metabolism Lysosomes / metabolism* Male Mercury / metabolism*, urine Mercury Poisoning / metabolism Nephrosis / metabolism Protein Binding Proteinuria / metabolism*, urine Rats |
| Chemical | |
Reg. No./Substance:
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0/Albumins; 7439-97-6/Mercury |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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