Document Detail


Menstrual cycle-related sialidase activity of the female cervical mucus is associated with exosome-like vesicles.
MedLine Citation:
PMID:  17562335     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To study endogenous sialidase activity in genital tract secretions of pregnant and nonpregnant women. DESIGN: Laboratory study. SETTING: Department of Evolutionary Biology and Department of Obstetrics and Reproductive Medicine, University of Siena, Siena, Italy. INTERVENTION(S): Vaginal and cervical mucus samples were obtained from pregnant and nonpregnant women in different phases of the menstrual cycle and in different weeks of pregnancy. MAIN OUTCOME MEASURE(S): Sialidase activity was assessed by fluorimetric assay and localized by transmission electron microscopy and differential centrifugation. RESULT(S): Sialidase activity in cervical mucus of healthy women reaches a maximum in the ovulatory phase. Cervical mucus from pregnant and nonpregnant women had significant sialidase activity that was associated with membranous vesicles having an exosome-like structure. CONCLUSION(S): Female cervical mucus contains an endogenous menstrual cycle-related sialidase that could be involved in modifying the rheologic properties of mucus to favor sperm progression at fertilization. Its association with exosome-like vesicles also suggests a role in intercellular communication before and after fertilization.
Authors:
Federica Flori; Federica Secciani; Antonietta Capone; Eugenio Paccagnini; Sabrina Caruso; Maria Grazia Ricci; Riccardo Focarelli
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-11
Journal Detail:
Title:  Fertility and sterility     Volume:  88     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-10-09     Completed Date:  2007-10-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1212-9     Citation Subset:  IM    
Affiliation:
Department of Evolutionary Biology, University of Siena, Policlinico le Scotte, Siena, Italy.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Cervix Mucus / enzymology*
Cytoplasmic Vesicles / enzymology*
Female
Humans
Menstrual Cycle / metabolism*
Middle Aged
Neuraminidase / metabolism*
Pregnancy
Chemical
Reg. No./Substance:
EC 3.2.1.18/Neuraminidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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